Lecak J, Scott K, Young C, Hannon J, Acker J P
Department of Laboratory Medicine and Pathology, University of Alberta, Alberta, Canada.
Transfusion. 2004 Sep;44(9):1306-13. doi: 10.1111/j.1537-2995.2004.03271.x.
RBCs frozen in 40 percent (wt/vol) glycerol are currently approved by the FDA and the AABB for storage at -80 degrees C for up to 10 years.
This study examined 20 RBC units that had been cryopreserved in 40 percent (wt/vol) glycerol and stored at -80 degrees C for up to 22 years. Measures of the freeze-thaw-wash (FTW) recovery, ATP, 2,3-DPG, methemoglobin, RBC indices, morphology, and osmotic fragility were made immediately after deglycerolization and after 24 hours of storage at 4 degrees C.
RBCs frozen for longer than 10 years had acceptable mean FTW recovery, normal oxygen transport function, RBC morphology, RBC indices, methemoglobin, and osmotic fragility. Statistical analysis indicated that the in-vitro viability and function of cryopreserved RBCs was not dependent on the length of frozen storage or postthaw storage at 4 degrees C but did correlate with the storage length at 4 degrees C before cryopreservation.
The data reported in this study demonstrate that RBCs can be stored at -80 degrees C beyond 10 years with acceptable in-vitro quality and suggest that more defined criteria for the cryopreservation process be adopted.
目前，美国食品药品监督管理局（FDA）和美国血库协会（AABB）批准在40%（重量/体积）甘油中冷冻保存的红细胞可在-80℃储存长达10年。
本研究检测了20个在40%（重量/体积）甘油中冷冻保存并在-80℃储存长达22年的红细胞单位。在去甘油化后以及在4℃储存24小时后，对冻融洗涤（FTW）回收率、三磷酸腺苷（ATP）、2,3-二磷酸甘油酸（2,3-DPG）、高铁血红蛋白、红细胞指数、形态和渗透脆性进行了检测。
冷冻超过10年的红细胞具有可接受的平均FTW回收率、正常的氧运输功能、红细胞形态、红细胞指数、高铁血红蛋白和渗透脆性。统计分析表明，冷冻保存红细胞的体外活力和功能不取决于冷冻储存时间或解冻后在4℃的储存时间，但与冷冻保存前在4℃的储存时间相关。
本研究报告的数据表明，红细胞可在-80℃储存超过10年，且体外质量可接受，并建议采用更明确的冷冻保存标准。
