已证实益生菌可改变鸟类胃肠道中的细菌群落结构。
Probiotics shown to change bacterial community structure in the avian gastrointestinal tract.
作者信息
Netherwood T, Gilbert H J, Parker D S, O'Donnell A G
机构信息
Department of Biological and Nutritional Sciences, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE1 7RU, Great Britain.
出版信息
Appl Environ Microbiol. 1999 Nov;65(11):5134-8. doi: 10.1128/AEM.65.11.5134-5138.1999.
Abstract
Culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (GI) tract following introduction of genetically modified (GM) and unmodified probiotics. Community hybridization of amplified 16S ribosomal DNA demonstrated that the bacterial flora of the GI tract changed significantly in response to the probiotic treatments. The changes were not detected by culturing. Although both GM and non-GM strains of Enterococcus faecium NCIMB 11508 changed the bacterial flora of the chicken GI tract, they did so differently. Probing the community DNA with an Enterococcus faecalis-specific probe showed that the relative amount of E. faecalis in the total eubacterial population increased in the presence of the non-GM strain and decreased in the presence of the GM probiotic compared with the results obtained with an untreated control group.
摘要
采用培养和分子技术监测引入转基因和非转基因益生菌后禽类胃肠道（GI）细菌菌群的变化。对扩增的16S核糖体DNA进行群落杂交表明，胃肠道细菌菌群因益生菌处理而发生显著变化。培养法未检测到这些变化。尽管屎肠球菌NCIMB 11508的转基因和非转基因菌株均改变了鸡胃肠道的细菌菌群，但方式不同。用粪肠球菌特异性探针探测群落DNA结果显示，与未处理对照组相比，在非转基因菌株存在时，总真细菌种群中粪肠球菌的相对数量增加，而在转基因益生菌存在时则减少。
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