Tdr2，一种新的Tc1家族斑马鱼转座子。

Tdr2, a new zebrafish transposon of the Tc1 family.

作者信息

Göttgens B, Barton L M, Grafham D, Vaudin M, Green A R

机构信息

Department of Haematology, Cambridge University, MRC Centre, Hills Road, Cambridge, UK.

出版信息

Gene. 1999 Nov 1;239(2):373-9. doi: 10.1016/s0378-1119(99)00390-x.

DOI:10.1016/s0378-1119(99)00390-x

PMID:10548740

Abstract

We describe here Tdr2, a new class of Tc1-like transposons in zebrafish. Tdr2 was identified from the genomic sequence of a zebrafish PAC (P1 artificial chromosome) clone, and fragments of Tdr2 were found in several zebrafish EST (expressed sequence tag) sequences. Predicted translation of the Tdr2 transposase gene showed that it was most closely related to Caenorhabditis elegans Tc3A, suggesting an ancient origin of the Tdr2 transposon. Tdr2 spans 1. 1kb and is flanked by inverted repeats of approx. 100bp. The 5' repeat is itself composed of an inverted repeat, raising the possibility of the formation of a cruciform DNA structure. Tdr2 transposons may facilitate the development of novel transposon-based tools for the genetic analysis of zebrafish.

摘要

我们在此描述Tdr2，这是斑马鱼中一类新的类Tc1转座子。Tdr2是从斑马鱼P1人工染色体（PAC）克隆的基因组序列中鉴定出来的，并且在多个斑马鱼表达序列标签（EST）序列中发现了Tdr2的片段。Tdr2转座酶基因的预测翻译表明，它与秀丽隐杆线虫的Tc3A关系最为密切，这表明Tdr2转座子起源古老。Tdr2全长1.1kb，两侧是约100bp的反向重复序列。5'端重复序列本身由一个反向重复序列组成，增加了形成十字形DNA结构的可能性。Tdr2转座子可能有助于开发用于斑马鱼遗传分析的新型基于转座子的工具。