Diğrak M, Ilçim A, Hakki Alma M
Department of Biology, Faculty of Arts and Science, Kahramanmaraş Sütçü Imam University, 46045-Kahramanmaraş, Turkey.
Phytother Res. 1999 Nov;13(7):584-7.
In this study, the antimicrobial activities of several parts of various trees grown in the Kahramanmaraş region of Turkey were investigated by the disc diffusion method. Chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of Pinus brutia Ten., Juniperus oxycedrus L., Abies cilicia Ant. & Kotschy Carr., Cedrus libani A. Rich. and Pinus nigra Arn. were prepared and tested against Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus cereus FMC 19, Escherichia coli DM, Klebsiella pneumoniae FMC 3, Enterobacter aerogenes CCM 2531, Staphylococcus aureus Cowan 1, Mycobacterium smegmatis RUT, Proteus vulgaris FMC 1, Listeria monocytogenes Scoot A, Pseudomonas aeruginosa DSM 5007, Candida albicans CCM 314, Candida tropicalis MDC 86 and Penicillium italicum K. The results showed that antifungal effects were not observed for the whole extracts, E. coli was not inhibited by any of the plant extracts except by the chloroform and acetone extracts of the leaves of A. cilicia, which showed inhibition zones of 16-18 mm, respectively. All the plant extracts used in this study inhibited the development of the other bacteria studied. When the results of this study were compared with an ampicillin standard, it was found that the microorganisms studied were generally susceptible, intermediate or resistant to the extracts of species when compared with the ampicillin standard. On the other hand, the acetone and methanol extracts of Juniperus fruits were found to be quite resistant.
在本研究中，采用纸片扩散法对生长在土耳其卡拉曼马拉什地区的多种树木的几个部位的抗菌活性进行了研究。制备了土耳其松、刺柏、塞浦路斯冷杉、黎巴嫩雪松和黑松的叶、树脂、树皮、球果和果实的氯仿、丙酮和甲醇提取物，并针对巨大芽孢杆菌DSM 32、枯草芽孢杆菌IMG 22、蜡样芽孢杆菌FMC 19、大肠杆菌DM、肺炎克雷伯菌FMC 3、产气肠杆菌CCM 2531、金黄色葡萄球菌科万1、耻垢分枝杆菌RUT、普通变形杆菌FMC 1、单核细胞增生李斯特菌Scoot A、铜绿假单胞菌DSM 5007、白色念珠菌CCM 314、热带念珠菌MDC 86和意大利青霉K进行了测试。结果表明，整个提取物未观察到抗真菌作用，除了塞浦路斯冷杉叶的氯仿和丙酮提取物分别显示出16 - 18毫米的抑菌圈外，任何植物提取物均未抑制大肠杆菌。本研究中使用的所有植物提取物均抑制了所研究的其他细菌的生长。当将本研究结果与氨苄青霉素标准进行比较时，发现所研究的微生物与氨苄青霉素标准相比，对各物种的提取物一般敏感、中度敏感或耐药。另一方面，发现刺柏果实的丙酮和甲醇提取物具有相当强的耐药性。
