Motazedian Mohammad Hossein, Mikaeili Fattaneh, Mohebali Mehdi, Miri Ramin, Habibi Parvaneh, Kamarloie Sakineh
Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Parasit Dis. 2017 Jun;41(2):375-379. doi: 10.1007/s12639-016-0809-y. Epub 2016 Jul 22.
Leishmaniasis is a parasitic disease caused by different species of protozoan parasites belonging to the genus . In this study, (MRHO/IR/75/ER) promastigotes were cultured at 23-25 °C in brain heart infusion BHI medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS) and penicillin and streptomycin. Antileishmanial effects of and methanolic extracts (0.07, 0.15, 0.31, 0.62, 1.25, 2.5, 5, 10 mg/mL) on promastigotes were evaluated using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. All experiments were repeated at least three times. methanolic extract did not show any activity while methanolic extract inhibited the growth of promastigote forms of in vitro after 72 h of incubation and had a 50 % inhibitory concentration (IC50) of 1.25 mg/mL. The methanolic extract of (henna) can be a promising antileishmanial agent in the future. Further experiments are needed for isolation of active fractions and identification of the active components of methanolic extract.
利什曼病是由属于利什曼原虫属的不同种类原生动物寄生虫引起的一种寄生虫病。在本研究中，（MRHO/IR/75/ER）前鞭毛体在补充有10%热灭活胎牛血清（FBS）以及青霉素和链霉素的脑心浸液（BHI）培养基中于23 - 25°C培养。使用MTT（3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐）法评估了[植物名称1]和[植物名称2]甲醇提取物（0.07、0.15、0.31、0.62、1.25、2.5、5、10mg/mL）对[寄生虫名称]前鞭毛体的抗利什曼作用。所有实验至少重复三次。[植物名称1]甲醇提取物未显示任何活性，而[植物名称2]甲醇提取物在孵育72小时后体外抑制了[寄生虫名称]前鞭毛体形式的生长，其50%抑制浓度（IC50）为1.25mg/mL。[植物名称2]（指甲花）的甲醇提取物未来可能是一种有前景的抗利什曼病药物。需要进一步实验来分离活性成分并鉴定甲醇提取物的活性成分。
