Yoshimoto M, Caminada De Toledo S R, Monteiro Caran E M, de Seixas M T, de Martino Lee M L, de Campos Vieira Abib S, Vianna S M, Schettini S T, Anderson Duffles Andrade J
Division of Genetics, Department of Morphology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
Am J Pathol. 1999 Nov;155(5):1439-43. doi: 10.1016/S0002-9440(10)65457-0.
Neuroblastoma is the second most common solid tumor occurring in children. Amplification of the MYCN oncogene is associated with poor prognosis. To identify neuroblastoma tumors with MYCN amplification, we studied the number of copies of MYCN in interphase cells by fluorescence in situ hybridization in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor specimens. Interphase and metaphase studies showed a tumor cell population with both forms of amplification, double minutes and homogeneously staining regions, in two patients. These patients showed a smaller tumor cell subpopulation with the presence of more than one homogeneously staining region, suggesting that gene amplification was undergoing karyotype evolution.
神经母细胞瘤是儿童中第二常见的实体瘤。MYCN癌基因的扩增与预后不良相关。为了识别具有MYCN扩增的神经母细胞瘤肿瘤，我们通过荧光原位杂交研究了20例神经母细胞瘤患者间期细胞中MYCN的拷贝数。7个肿瘤标本中出现了MYCN扩增。间期和中期研究显示，两名患者的肿瘤细胞群体同时存在两种扩增形式，即双微体和均匀染色区。这些患者显示出一个较小的肿瘤细胞亚群，存在一个以上的均匀染色区，这表明基因扩增正在经历核型进化。
