采用可见/近红外反射光谱法同时定量分析西洋参（Panax quinquefolium）根粉中的人参皂苷。

Simultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near-infrared reflectance spectroscopy.

作者信息

Ren G, Chen F

机构信息

Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong.

出版信息

J Agric Food Chem. 1999 Jul;47(7):2771-5. doi: 10.1021/jf9812477.

DOI:10.1021/jf9812477

PMID:10552563

Abstract

Near-infrared reflectance spectroscopy (NIRS) was examined as a possible alternative to high-performance liquid chromatography (HPLC) for the analysis of ginsenosides from American ginseng (Panax quinquefolium) root powder (n = 26). NIR spectra were collected over 400-2500 nm. For each sample and individual ginsenoside quantified by HPLC, spectral data were regressed against the chemical data to develop prediction equations. The spectral prediction equations produced high correlation coefficient (1-VR) values and low standard errors of cross validation (SECV) values for the determination of individual and total ginsenosides. The contents of individual ginsenosides, Rb(1), Rb(2), Rc, Rd, Re, Rg(1), Ro, m-Rb(1), m-Rb(2), m-Rc, m-Rd, and total ginsenosides (X +/- SECV) were (1.29+/-0.18)%, (0.273+/-0.096)%, (0.298+/-0.052)%, (0.091+/-0.027)%, (1. 015+/-0.114)%, (0.116+/-0.018)%, (0.25+/-0.040)%, (0.776+/-0.116)%, (0.197+/-0.074)%, (0.239+/-0.083)%, (0.143+/-0.042)%, and (4.393+/-0.283)%, respectively. The (1-VR) values of cross validation were 0.877, 0.872, 0.955, 0.834, 0.899, 0.919, 0.325, 0.849, 0.902, 0.877, 0.871, and 0.963, respectively. Results indicated that the NIRS method could be used for the analysis of the major ginsenosides, Rb(1), Re, and m-Rb(1), as well as the total ginsenosides in American ginseng.

摘要

研究了近红外反射光谱法（NIRS）作为高效液相色谱法（HPLC）的一种可能替代方法，用于分析西洋参（Panax quinquefolium）根粉中的人参皂苷（n = 26）。在400 - 2500 nm范围内收集近红外光谱。对于通过HPLC定量的每个样品和每种人参皂苷，将光谱数据与化学数据进行回归以建立预测方程。光谱预测方程在测定单个和总人参皂苷时产生了高相关系数（1-VR）值和低交叉验证标准误差（SECV）值。单个人参皂苷Rb(1)、Rb(2)、Rc、Rd、Re、Rg(1)、Ro、m-Rb(1)、m-Rb(2)、m-Rc、m-Rd以及总人参皂苷（X +/- SECV）的含量分别为(1.29 +/- 0.18)%、(0.273 +/- 0.096)%、(0.298 +/- 0.052)%、(0.091 +/- 0.027)%、(1.015 +/- 0.114)%、(0.116 +/- 0.018)%、(0.25 +/- 0.040)%、(0.776 +/- 0.116)%、(0.197 +/- 0.074)%、(0.239 +/- 0.083)%、(0.143 +/- 0.042)%和(4.393 +/- 0.283)%。交叉验证的（1-VR）值分别为0.877、0.872、0.955、0.834、0.899、0.919、0.325、0.849、0.902、0.877、0.871和0.963。结果表明，近红外光谱法可用于分析西洋参中的主要人参皂苷Rb(1)、Re和m-Rb(1)以及总人参皂苷。