用于确定单纯疱疹病毒临床分离株体外敏感性的快速筛查试验。
Rapid screening tests for determining in vitro susceptibility of herpes simplex virus clinical isolates.
作者信息
de la Iglesia P, Melón S, López B, Rodriguez M, Blanco M I, Mellado P, de Oña M
机构信息
Laboratorio de Virología, Servicio de Microbiologia, Hospital, Asturias, Spain.
出版信息
J Clin Microbiol. 1998 Aug;36(8):2389-91. doi: 10.1128/JCM.36.8.2389-2391.1998.
The susceptibility of human herpes simplex virus (HSV) to acyclovir (ACV) was determined with the use of a single dose of the drug (1 and 2 micrograms of ACV per ml for HSV-1 and HSV-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (Rapid CIA) and a rapid dye uptake assay (Rapid DUA). These tests allow the simultaneous determination of virus titer and susceptibility to ACV at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye uptake units). These tests were compared with a conventional susceptibility assay (dye uptake assay) and showed similar results. Indeterminate results with the Rapid CIA appeared in 3 of 30 samples. With the use of both Rapid CIA and Rapid DUA, we were able to determine the susceptibility of 100% of the isolates. The rapid tests, unlike conventional assays, are able to provide susceptibility results within 3 days after the virus has been isolated from a clinical specimen and could thus play a direct role in therapeutic decisions.
使用单剂量药物（分别针对单纯疱疹病毒1型和2型，每毫升含1微克和2微克阿昔洛韦），通过两种快速检测方法来测定人单纯疱疹病毒（HSV）对阿昔洛韦（ACV）的敏感性：快速细胞病变效应抑制检测法（快速CIA）和快速染料摄取检测法（快速DUA）。这些检测方法可在确定的病毒浓度（100个50%组织培养感染剂量和100个50%染料摄取单位）下同时测定病毒滴度和对阿昔洛韦的敏感性。将这些检测方法与传统敏感性检测法（染料摄取检测法）进行比较，结果相似。30个样本中有3个样本的快速CIA结果不确定。同时使用快速CIA和快速DUA，我们能够确定100%分离株的敏感性。与传统检测方法不同，快速检测方法能够在从临床标本中分离出病毒后的3天内提供敏感性结果，因此可以在治疗决策中发挥直接作用。
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