Plant Biology Program, Department of Biology, Washington University, 63130, St. Louis, MO, USA.
Planta. 1981 Oct;153(2):130-9. doi: 10.1007/BF00384094.
Mature seeds of Glycine max (L.) Merr. contain two major storage proteins, a glycosylated 7S protein (conglycinin) and a non-glycosylated 11S protein (glycinin). Accumulation of these proteins and their mRNAs during seed development in cv. Provar was studied by SDS polyacrylamide gel electrophoresis and by "Northern" (DNA-RNA) hybridization. The 11S acidic and basic subunits and the 7S α' and α subunits began to accumulate 18-20 d after pollination, shortly after the termination of cell division in developing cotyledons, whereas the 7S β and 11S A-4 subunits were not detected until one to two weeks later, during the maturation phase of development. Messenger RNAs for 7S and 11S proteins were first detected 14-18 d after pollination, several days before the accumulation of storage proteins. Extracts from embryonic axes contained reduced levels of the 7S α subunit, very little 11S protein, no detectable 7S β or 11S A-4 subunits, and an additional 7S subunit not found in cotyledons. Soybean axes and cotyledons therefore differ in their synthesis of seed storage proteins.
成熟的大豆种子（Glycine max（L.）Merr.）含有两种主要的储存蛋白，一种是糖基化的 7S 蛋白（伴大豆球蛋白）和一种是非糖基化的 11S 蛋白（大豆球蛋白）。通过 SDS 聚丙烯酰胺凝胶电泳和“Northern”（DNA-RNA）杂交研究了 cv. Provar 种子发育过程中这些蛋白质及其 mRNA 的积累。11S 酸性和碱性亚基以及 7S α'和α亚基在授粉后 18-20 天开始积累，此时发育中的子叶细胞分裂结束后不久，而 7S β和 11S A-4 亚基直到一到两周后才被检测到，此时是发育的成熟阶段。7S 和 11S 蛋白的 mRNA 是在授粉后 14-18 天首次检测到的，比储存蛋白积累早几天。胚胎轴提取物中 7S α 亚基的水平降低，11S 蛋白含量很少，7S β或 11S A-4 亚基无法检测到，并且还存在一种在子叶中未发现的额外 7S 亚基。因此，大豆轴和子叶在种子储存蛋白的合成上存在差异。
