Dottin R P, Cutler L S, Pearson M L
Proc Natl Acad Sci U S A. 1975 Mar;72(3):804-8. doi: 10.1073/pnas.72.3.804.
Purified lambda repressor protein is shown to reduce the lambda DNA-directed synthesis of proteins in vitro as determined both by net amino-acid incorporation and by analysis of specific lambda-coded proteins resolved by sodium dodecyl sulfate/polyacrylamide slab gel electrophoresis. By means of different lambda DNA templates carrying deletion and point mutations in the operators o-L or o-R, it has been possible to demonstrate repression of the synthesis of two classes of lambda proteins. The synthesis of one, class c, appears to be controlled from the operator o-L and is more efficiently repressed at low concentrations of the repressor than that of the other class of repressible lambda proteins, class d, which is controlled from the operator o-R. Several other proteins synthesized in vitro are not repressible. Some of these are coded by the J-att region. In addition, the repressor appears to have another activity, that of stimulating the synthesis of a protein identified as the repressor itself. Lambda repressor appears to stimulate its own synthesis by acting at prm, a site defined by the cis-acting mutation prm 116.
纯化的λ阻遏蛋白在体外可降低由λDNA指导的蛋白质合成，这一点通过净氨基酸掺入以及对经十二烷基硫酸钠/聚丙烯酰胺平板凝胶电泳分离的特定λ编码蛋白的分析得以确定。借助在操纵基因o-L或o-R中携带缺失和点突变的不同λDNA模板，已能够证明对两类λ蛋白合成的阻遏作用。其中一类，即c类蛋白的合成，似乎受操纵基因o-L控制，并且在阻遏蛋白低浓度时比另一类可阻遏的λ蛋白（d类蛋白，受操纵基因o-R控制）的合成更有效地受到抑制。体外合成的其他几种蛋白质不可被阻遏。其中一些由J-att区域编码。此外，阻遏蛋白似乎还有另一种活性，即刺激一种被鉴定为阻遏蛋白自身的蛋白质的合成。λ阻遏蛋白似乎通过作用于prm来刺激自身合成，prm是由顺式作用突变prm 116定义的一个位点。
