Hofman-Bang J
Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark.
Mol Biotechnol. 1999 Aug;12(1):35-73. doi: 10.1385/MB:12:1:35.
In Saccharomyces cerevisiae the expression of all known nitrogen catabolite pathways are regulated by four regulators known as Gln3, Gat1, Dal80, and Deh1. This is known as nitrogen catabolite repression (NCR). They bind to motifs in the promoter region to the consensus sequence 5'GATAA 3'. Gln3 and Gat1 act positively on gene expression whereas Dal80 and Deh1 act negatively. Expression of nitrogen catabolite pathway genes known to be regulated by these four regulators are glutamine, glutamate, proline, urea, arginine. GABA, and allantonie. In addition, the expression of the genes encoding the general amino acid permease and the ammonium permease are also regulated by these four regulatory proteins. Another group of genes whose expression is also regulated by Gln3, Gat1, Dal80, and Deh1 are some proteases, CPS1, PRB1, LAP1, and PEP4, responsible for the degradation of proteins into amino acids thereby providing a nitrogen source to the cell. In this review, all known promoter sequences related to expression of nitrogen catabolite pathways are discussed as well as other regulatory proteins. Overview of metabolic pathways and promotors are presented.
在酿酒酵母中，所有已知的氮分解代谢途径的表达都受四种调控因子Gln3、Gat1、Dal80和Deh1的调控。这被称为氮分解代谢物阻遏（NCR）。它们与启动子区域中与共有序列5'GATAA 3'结合的基序相结合。Gln3和Gat1对基因表达起正向作用，而Dal80和Deh1起负向作用。已知受这四种调控因子调控的氮分解代谢途径基因有谷氨酰胺、谷氨酸、脯氨酸、尿素、精氨酸、γ-氨基丁酸和尿囊素。此外，编码通用氨基酸通透酶和铵通透酶的基因表达也受这四种调控蛋白的调控。另一组其表达也受Gln3、Gat1、Dal80和Deh1调控的基因是一些蛋白酶，如CPS1、PRB1、LAP1和PEP4，它们负责将蛋白质降解为氨基酸，从而为细胞提供氮源。在本综述中，讨论了所有已知的与氮分解代谢途径表达相关的启动子序列以及其他调控蛋白。还介绍了代谢途径和启动子的概述。
