Servicio Regional de Investigación y Desarrollo Agroalimentario SERIDA, Principado de Asturias, 33300 Villaviciosa, Spain.
Syst Appl Microbiol. 2013 Jul;36(5):320-4. doi: 10.1016/j.syapm.2013.04.004. Epub 2013 May 30.
Five strains of gram negative bacteria, isolated from soybean (LPPA 221(T), 222 and 223) and weeds (LPPA 816 and 1442), were analyzed by a polyphasic approach. The isolates showed variation in their phenotypic traits and were placed in the Pseudomonas fluorescens lineage, based on 16S rRNA gene sequence phylogeny, as a single but well separated cluster. MLSA analysis based on gyrB and rpoD sequences clustered the strains in a single branch in the Pseudomonas syringae group, and revealed P. viridiflava as closest relative. DNA-DNA hybridizations showed medium levels of DNA-DNA relatedness with the type strain of P. viridiflava (50%) and lower levels (<32%) with other type strains of the P. syringae group, supporting classification within a novel species of the genus Pseudomonas. The strains can be distinguished from species of the P. syringae group by the fatty acid C17:0 cyclo that is present in a low amount (2.5%) and from P. viridiflava by their inability to assimilate d-tartrate and d-sorbitol, and by the formation of red colonies on TTC medium. For this new species, the name Pseudomonas asturiensis sp. nov. is proposed. The type strain is LPPA 221(T) (=LMG 26898(T)=CECT 8095(T)).
从大豆（LPPA 221(T)、222 和 223）和杂草（LPPA 816 和 1442）中分离出的五株革兰氏阴性细菌，通过多相分析进行了分析。根据 16S rRNA 基因序列系统发育，这些分离株在表型特征上存在差异，被归为荧光假单胞菌谱系，形成一个单一但分离良好的聚类。基于 gyrB 和 rpoD 序列的 MLSA 分析将菌株聚类为丁香假单胞菌群的一个分支，并且发现绿脓假单胞菌是最接近的亲缘关系。DNA-DNA 杂交显示与绿脓假单胞菌的模式株具有中等水平的 DNA-DNA 相关性（50%），与丁香假单胞菌群的其他模式株的相关性较低（<32%），支持在假单胞菌属内分类为一个新种。这些菌株可以通过存在低量（2.5%）的 C17:0 环脂肪酸与丁香假单胞菌群的物种区分开来，并且不能同化 d-酒石酸盐和 d-山梨醇，以及在 TTC 培养基上形成红色菌落与绿脓假单胞菌区分开来。对于这个新物种，建议使用 Pseudomonas asturiensis sp. nov. 这个名称。模式菌株是 LPPA 221(T)（=LMG 26898(T)=CECT 8095(T)）。
