Barbier G, Godfroy A, Meunier J R, Quérellou J, Cambon M A, Lesongeur F, Grimont P A, Raguénès G
Laboratoire de Caractérisation des Microorganismes Marins, IFREMER, Centre de Brest, Plouzané, France.
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1829-37. doi: 10.1099/00207713-49-4-1829.
A hyperthermophilic archaeon, strain AL585T, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at latitude 13 degrees N and a depth of 2650 m. The isolate was a strictly anaerobic coccus with a mean cell diameter of 1 micron. The optimum temperature, pH and concentration of sea salt for growth were 95 degrees C, 7.5 and 30 g l-1. Under these conditions, the doubling time and cell yield were 0.5 h and 5 x 10(8) cells ml-1. Strain AL585T grew preferentially in media containing complex proteinaceous carbon sources, glucose and elemental sulfur. The G + C content of the DNA was 47 mol%. Sequencing of the 16S rDNA gene showed that strain AL585T belonged to the genus Pyrococcus and was probably a new species. This was confirmed by total DNA hybridization. Consequently, this strain is described as a new species, Pyrococcus glycovorans sp. nov.
从位于北纬13度、深度为2650米的东太平洋海隆的深海热液喷口中分离出一株嗜热古菌，菌株AL585T。该分离株是一种严格厌氧的球菌，平均细胞直径为1微米。生长的最适温度、pH值和海盐浓度分别为95℃、7.5和30 g l-1。在这些条件下，倍增时间和细胞产量分别为0.5小时和5×10(8)个细胞ml-1。菌株AL585T优先在含有复合蛋白质碳源、葡萄糖和元素硫的培养基中生长。DNA的G + C含量为47 mol%。16S rDNA基因测序表明，菌株AL585T属于嗜热栖热菌属，可能是一个新物种。这通过总DNA杂交得到证实。因此，该菌株被描述为一个新物种，嗜热糖栖热菌（Pyrococcus glycovorans sp. nov.）。
