Kecha Mouloud, Benallaoua Said, Touzel Jean Pierre, Bonaly Roger, Duchiron Francis
Laboratoire de Microbiologie Industrielle, URCA Université de Reims Champagne Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 02, France.
Extremophiles. 2007 Jan;11(1):65-73. doi: 10.1007/s00792-006-0010-9. Epub 2006 Sep 13.
A hyperthermophilic anaerobic archeon, strain HT3, was isolated from hydrothermal hot spring in Northeast Algeria. The strain is a regular coccus, highly motile, obligatory anaerobic, heterotrophic. It utilizes proteinaceous complex media (peptone, tryptone or yeast extract). Sulfur is reduced to Hydrogen sulfide and enhances growth. It shares with other Pyrococcus species the heterotrophic mode of nutrition, the hyperthermophily, the ability to utilize amino acids as sole carbon and nitrogen sources and the ether lipid composition. The optimal growth occurs at 80-85 degrees C, pH 7.5 and 1.5% NaCl. The G + C content was 43 mol%. Considering its morphology, physiological properties, nutritional features and phylogenetic analyses based on 16S rRNA gene sequencing, this strain is described as a new terrestrial isolate pertaining to the genus Pyrococcus.
从阿尔及利亚东北部的热液温泉中分离出了一种嗜热厌氧古菌，HT3菌株。该菌株为规则球菌，运动性强， obligatory厌氧，异养型。它利用含蛋白质的复合培养基（蛋白胨、胰蛋白胨或酵母提取物）。硫被还原为硫化氢并促进生长。它与其他火球菌属物种具有异养营养模式、嗜热特性、将氨基酸作为唯一碳源和氮源利用的能力以及醚脂组成。最佳生长温度为80 - 85摄氏度，pH值为7.5，NaCl浓度为1.5%。G + C含量为43摩尔%。基于其形态、生理特性、营养特征以及基于16S rRNA基因测序的系统发育分析，该菌株被描述为火球菌属的一种新的陆地分离株。 （注：原文中“obligatory anaerobic”这里的“obligatory”可能有误，推测可能是“obligate”，意为专性厌氧 ）
