Dennis A J, Wilson H E
Ann N Y Acad Sci. 1975 Jan 27;243:73-80. doi: 10.1111/j.1749-6632.1975.tb25345.x.
Peripheral lymphocytes from patients with chronic lymphocytic leukemia display a delayed and lowered response to stimulation by PHA, as compared with the lymphocytes from normal donors. This response was determined by measuring the incorporation of tritiated thymidine. The addition of DMSO in concentrations of 0.1%, 0.25%, 0.5%, 1.0%, and 2.0% (v/v) to the culture fluid of normal cell cultures that had been stimulated, with PHA elicited a 3- to 4-fold increase in the rate of incorporation of labeled thymidine at the 3-day test period. The addition of 2.0% DMSO proved to be preferentially cytotoxic to cultures of leukemic cells after 48 hours, as compared with the virtual absence of cytotoxicity for cultures of normal lymphocytes. The addition of 0.1%, 0.25%, 0.5%, and 1.0% DMSO to cultures of leukemic cells stimulated with PHA (as were normal cell cultures) resulted in a 6- to 6.5-fold increase in the rate of uptake of tritiated thymidine at 3 days.
与正常供体的淋巴细胞相比，慢性淋巴细胞白血病患者的外周淋巴细胞对PHA刺激的反应延迟且降低。这种反应通过测量氚标记胸腺嘧啶核苷的掺入来确定。向已用PHA刺激的正常细胞培养物的培养液中添加浓度为0.1%、0.25%、0.5%、1.0%和2.0%（v/v）的DMSO，在3天测试期时，标记胸腺嘧啶核苷的掺入率提高了3至4倍。与对正常淋巴细胞培养物几乎没有细胞毒性相比，添加2.0%DMSO在48小时后对白血病细胞培养物具有优先细胞毒性。向用PHA刺激的白血病细胞培养物（与正常细胞培养物一样）中添加0.1%、0.25%、0.5%和1.0%DMSO，在3天时氚标记胸腺嘧啶核苷的摄取率提高了6至6.5倍。
