Hevener W, Routh P A, Almond G W
College of Veterinary Medicine, North Carolina State University, Raleigh 27606-1499, USA.
Can Vet J. 1999 Nov;40(11):782-6.
This study was designed to determine the long-term effects of repeated endotoxin treatment or immunization against human serum albumin on concentrations of serum insulin-like growth factor-I (IGF-I) and other indicators of growth performance in growing pigs. Thirty gilts (38.5 +/- 0.9 kg) were randomly assigned to 5 treatment groups (n = 6 animals/group): 1) lipopolysaccharide injections, 2) lipopolysaccharide pair-fed, 3) human serum albumin immunization, 4) human serum albumin pair-fed, and 5) control. Pigs in the lipopolysaccharide group were treated intramuscularly with lipopolysaccharide on Days 0-3. The pigs in the human serum albumin group were immunized with human serum albumin emulsified in Freund's adjuvant on Day 0 and administered a booster on Day 28. The lipopolysaccharide pair-fed pigs were matched by body weight and pair-wise fed with pigs treated with lipopolysaccharide. Similarly, human serum albumin pair-fed pigs were matched to human serum albumin immunized pigs. Serum IGF-I concentrations did not differ between or within groups. There was no difference in feed disappearance between groups prior to the initiation of treatments. The lipopolysaccharide group had a decrease (P = 0.013) in feed disappearance on Day 0 compared with control and human serum albumin groups. On Day 1, both lipopolysaccharide and human serum albumin groups differed (P < 0.05) from control. Average daily gain and total weight gain did not differ between groups; however, feed efficiency differed (P < 0.05) between lipopolysaccharide and control groups. Long-term effects of repeated endotoxin challenge or immunization on IGF-I concentrations and growth were not evident in the present study. This failure presumably was due to the development of endotoxin tolerance and a relatively innocuous vaccination against human serum albumin.
本研究旨在确定反复内毒素处理或针对人血清白蛋白免疫对生长猪血清胰岛素样生长因子-I（IGF-I）浓度及其他生长性能指标的长期影响。30头后备母猪（38.5±0.9千克）被随机分为5个处理组（每组n = 6头动物）：1）脂多糖注射组，2）脂多糖配对饲养组，3）人血清白蛋白免疫组，4）人血清白蛋白配对饲养组，5）对照组。脂多糖组的猪在第0至3天接受肌肉注射脂多糖。人血清白蛋白组的猪在第0天用弗氏佐剂乳化的人血清白蛋白进行免疫，并在第28天进行加强免疫。脂多糖配对饲养的猪按体重匹配，并与接受脂多糖处理的猪成对饲养。同样，人血清白蛋白配对饲养的猪与接受人血清白蛋白免疫的猪匹配。各处理组之间及组内血清IGF-I浓度无差异。在处理开始前，各处理组之间的采食量没有差异。与对照组和人血清白蛋白组相比，脂多糖组在第0天的采食量下降（P = 0.013）。在第1天，脂多糖组和人血清白蛋白组均与对照组不同（P < 0.05）。各处理组之间的平均日增重和总增重无差异；然而，脂多糖组与对照组之间的饲料效率不同（P < 0.05）。在本研究中，反复内毒素攻击或免疫对IGF-I浓度和生长的长期影响并不明显。这种失败可能是由于内毒素耐受性的发展以及针对人血清白蛋白的相对无害的疫苗接种。
