来自印度楝的具有生物活性的咔唑生物碱。

Biologically active carbazole alkaloids from Murraya koenigii.

作者信息

Ramsewak R S, Nair M G, Strasburg G M, DeWitt D L, Nitiss J L

机构信息

Department of Horticulture and National Food Safety and Toxicology Center, Michigan State University, East Lansing 48824, USA.

出版信息

J Agric Food Chem. 1999 Feb;47(2):444-7. doi: 10.1021/jf9805808.

DOI:10.1021/jf9805808

PMID:10563914

Abstract

The bioassay guided fractionation of the acetone extract of the fresh leaves of Murraya koenigii resulted in the isolation of three bioactive carbazole alkaloids, mahanimbine (1), murrayanol (2), and mahanine (3), as confirmed from their (1)H and (13)C NMR spectral data. Compound 2 showed an IC(50) of 109 microg/mL against hPGHS-1 and an IC(50) of 218 microg/mL against hPGHS-2 in antiinflammatory assays, while compound 1 displayed antioxidant activity at 33.1 microg/mL. All three compounds were mosquitocidal and antimicrobial and exhibited topoisomerase I and II inhibition activities.

摘要

对九里香鲜叶丙酮提取物进行生物测定导向的分级分离，得到了三种生物活性咔唑生物碱，即马汉九里香碱（1）、九里香醇（2）和马汉碱（3），这通过它们的氢核磁共振（¹H NMR）和碳核磁共振（¹³C NMR）光谱数据得到证实。在抗炎试验中，化合物2对人前列腺素内过氧化物合酶-1（hPGHS-1）的半数抑制浓度（IC₅₀）为109微克/毫升，对人前列腺素内过氧化物合酶-2（hPGHS-2）的IC₅₀为218微克/毫升，而化合物1在33.1微克/毫升时表现出抗氧化活性。所有三种化合物都具有杀蚊和抗菌活性，并表现出对拓扑异构酶I和II的抑制活性。