在意大利锡耶纳地区的一次疫情期间，通过巢式聚合酶链反应快速鉴定托斯卡纳病毒。
Rapid identification of Toscana virus by nested PCR during an outbreak in the Siena area of Italy.
作者信息
Valassina M, Cusi M G, Valensin P E
机构信息
Department of Molecular Biology, University of Siena, Italy.
出版信息
J Clin Microbiol. 1996 Oct;34(10):2500-2. doi: 10.1128/jcm.34.10.2500-2502.1996.
Abstract
The sand fly-transmitted Toscana virus is recognized as an etiologic agent of an aseptic meningitis with a long convalescence. This infection has been reported overall in many tourists or in a seronegative population circulating in endemic Mediterranean areas (Italy, Portugal, Egypt, and Cyprus). We report a cluster of acute Toscana virus infections in the local population during the summer of 1995. Twenty-one clinical cases of meningitis were investigated for the presence of Toscana virus by nested PCR performed on the S segment of the virus RNA extracted from cerebrospinal fluid samples.
摘要
白蛉传播的托斯卡纳病毒被认为是一种导致无菌性脑膜炎且恢复期较长的病原体。总体而言，这种感染在许多游客或在地中海流行地区（意大利、葡萄牙、埃及和塞浦路斯）流动的血清阴性人群中均有报道。我们报告了1995年夏季当地人群中发生的一组急性托斯卡纳病毒感染病例。通过对从脑脊液样本中提取的病毒RNA的S片段进行巢式PCR，对21例脑膜炎临床病例进行了托斯卡纳病毒检测。
相似文献
1
Rapid identification of Toscana virus by nested PCR during an outbreak in the Siena area of Italy.在意大利锡耶纳地区的一次疫情期间，通过巢式聚合酶链反应快速鉴定托斯卡纳病毒。
J Clin Microbiol. 1996 Oct;34(10):2500-2. doi: 10.1128/jcm.34.10.2500-2502.1996.
2
Toscana virus infections in northern Italy: laboratory and clinical evaluation.意大利北部的托斯卡纳病毒感染：实验室和临床评估。
Vector Borne Zoonotic Dis. 2012 Jun;12(6):526-9. doi: 10.1089/vbz.2011.0781. Epub 2012 Mar 26.
3
Evidence of Toscana virus variants circulating in Tuscany, Italy, during the summers of 1995 to 1997.1995年至1997年夏季期间，意大利托斯卡纳地区存在托斯卡纳病毒变种传播的证据。
J Clin Microbiol. 1998 Jul;36(7):2103-4. doi: 10.1128/JCM.36.7.2103-2104.1998.
4
Detection of neurotropic viruses circulating in Tuscany: the incisive role of Toscana virus.托斯卡纳地区循环的嗜神经病毒检测：托斯卡纳病毒的关键作用。
J Med Virol. 2000 Jan;60(1):86-90. doi: 10.1002/(sici)1096-9071(200001)60:1<86::aid-jmv14>3.0.co;2-n.
5
Acute meningitis due to Toscana virus infection among patients from both the Spanish Mediterranean region and the region of Madrid.西班牙地中海地区和马德里地区患者中因托斯卡纳病毒感染所致的急性脑膜炎。
J Clin Virol. 2003 Jan;26(1):79-84. doi: 10.1016/s1386-6532(02)00041-0.
6
Toscana virus meningitis in Portugal, 2002-2005.2002 - 2005年葡萄牙的托斯卡纳病毒脑膜炎
Euro Surveill. 2007 Jun 1;12(6):E3-4. doi: 10.2807/esm.12.06.00715-en.
7
Epidemiology of Toscana virus in South Tuscany over the years 2011-2019.2011 年至 2019 年托斯卡纳病毒在南托斯卡纳的流行病学研究。
J Clin Virol. 2020 Jul;128:104452. doi: 10.1016/j.jcv.2020.104452. Epub 2020 May 22.
8
Central nervous system involvement during infection by Phlebovirus toscana of residents in natural foci in central Italy (1977-1988).意大利中部自然疫源地居民感染托斯卡纳白蛉病毒期间的中枢神经系统受累情况（1977 - 1988年）
Am J Trop Med Hyg. 1991 Oct;45(4):429-34. doi: 10.4269/ajtmh.1991.45.429.
9
Meningitis and epididymitis caused by Toscana virus infection imported to Switzerland diagnosed by metagenomic sequencing: a case report.瑞士输入性土库曼斯坦病毒感染引起的脑膜炎和附睾炎的宏基因组测序诊断：病例报告。
BMC Infect Dis. 2019 Jul 8;19(1):591. doi: 10.1186/s12879-019-4231-9.
10
[Presence of the Toscana virus in Majorca. Prevalence and epidemiological characteristics in a hospital population].[托斯卡纳病毒在马略卡岛的存在情况。医院人群中的流行率及流行病学特征]
Enferm Infecc Microbiol Clin. 2011 Apr;29(4):315-6. doi: 10.1016/j.eimc.2010.10.004. Epub 2011 Feb 23.
引用本文的文献
1
Toscana virus - an emerging Mediterranean arbovirus transmitted by sand flies.托斯卡纳病毒——一种由沙蝇传播的新兴地中海虫媒病毒。
J Gen Virol. 2024 Nov;105(11). doi: 10.1099/jgv.0.002045.
2
[Sandfly fever-a "neglected" disease].[白蛉热——一种“被忽视的”疾病]
Hautarzt. 2018 Nov;69(11):928-937. doi: 10.1007/s00105-018-4251-1.
3
Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein.结构洞察托斯卡纳病毒核蛋白的 RNA 包装和螺旋组装。
Nucleic Acids Res. 2014 May;42(9):6025-37. doi: 10.1093/nar/gku229. Epub 2014 Mar 31.
4
Emergence of Toscana virus in the mediterranean area.托斯卡纳病毒在地中海地区的出现。
World J Virol. 2012 Oct 12;1(5):135-41. doi: 10.5501/wjv.v1.i5.135.
5
Immunoglobulin M seropositivity for Toscana virus in a random population sample in Sicily.西西里岛随机人群样本中抗托斯卡纳病毒免疫球蛋白 M 阳性。
Int J Infect Dis. 2012 Aug;16(8):e633-5. doi: 10.1016/j.ijid.2012.04.012. Epub 2012 Jun 21.
6
Seroprevalence of and risk factors for Toscana and Sicilian virus infection in a sample population of Sicily (Italy).西西里岛（意大利）人群中托斯卡纳病毒和西西里病毒感染的血清流行率及危险因素。
J Infect. 2012 Feb;64(2):212-7. doi: 10.1016/j.jinf.2011.11.012. Epub 2011 Nov 18.
7
Prevalence of toscana and sicilian phlebovirus antibodies in classic Kaposi sarcoma case patients and control subjects in sicily.西西里经典卡波西肉瘤病例患者和对照受试者中托斯卡纳和西西里静脉病毒抗体的流行情况。
J Infect Dis. 2011 Nov;204(9):1423-6. doi: 10.1093/infdis/jir546. Epub 2011 Sep 7.
8
Age-dependent seroprevalence of Toscana virus in central Italy and correlation with the clinical profile.意大利中部托斯卡纳病毒的年龄依赖性血清阳性率及其与临床特征的相关性。
Clin Vaccine Immunol. 2009 Aug;16(8):1251-2. doi: 10.1128/CVI.00376-08. Epub 2009 Jun 24.
9
Toscana virus RNA in Sergentomyia minuta files.微小真蚋中托斯卡纳病毒RNA
Emerg Infect Dis. 2006 Aug;12(8):1299-300. doi: 10.3201/eid1708.060345.
10
Toscana virus central nervous system infections in southern Italy.意大利南部托斯卡纳病毒引起的中枢神经系统感染
J Clin Microbiol. 2005 Dec;43(12):6186-8. doi: 10.1128/JCM.43.12.6186-6188.2005.
本文引用的文献
1
Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.无菌性脑膜炎患者标本中肠道病毒的快速灵敏检测
J Clin Microbiol. 1996 Jan;34(1):199-201. doi: 10.1128/jcm.34.1.199-201.1996.
2
Toscana virus genomic L segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand RNA viruses.托斯卡纳病毒基因组L片段：与其他负链RNA病毒相比的分子克隆、编码策略及氨基酸序列
Virus Res. 1993 Feb;27(2):119-31. doi: 10.1016/0168-1702(93)90076-y.
3
Nested PCR for specific detection and rapid identification of human picornaviruses.用于特异性检测和快速鉴定人微小核糖核酸病毒的巢式聚合酶链反应
J Clin Microbiol. 1994 Feb;32(2):285-91. doi: 10.1128/jcm.32.2.285-291.1994.
4
Travel-related Toscana virus infection.与旅行相关的托斯卡纳病毒感染
Lancet. 1993 Sep 25;342(8874):803-4. doi: 10.1016/0140-6736(93)91568-7.
5
Serosurvey and laboratory diagnosis of imported sandfly fever virus, serotype Toscana, infection in Germany.德国输入性托斯卡纳型白蛉热病毒感染的血清学调查及实验室诊断
Epidemiol Infect. 1995 Jun;114(3):501-10. doi: 10.1017/s0950268800052213.
6
7
Rheumatoid factors as a cause for false positive IgM anti-toxoplasma fluorescent tests. A technique for specific results.类风湿因子导致IgM抗弓形虫荧光试验假阳性。一种获得特异性结果的技术。
Rev Inst Med Trop Sao Paulo. 1972 Sep-Oct;14(5):310-3.
8
Toscana virus infection in United States citizen returning from Italy.一名从意大利返回的美国公民感染托斯卡纳病毒。
Lancet. 1987 Jan 17;1(8525):165-6. doi: 10.1016/s0140-6736(87)92005-8.
9
Neurovirulent Toscana virus (a sandfly fever virus) in Swedish man after visit to Portugal.一名瑞典男子在访问葡萄牙后感染了神经毒性托斯卡纳病毒（一种白蛉热病毒）。
Lancet. 1985 May 25;1(8439):1212-3. doi: 10.1016/s0140-6736(85)92886-7.
10
The genus Phlebovirus and its vectors.白蛉病毒属及其传播媒介。
Annu Rev Entomol. 1988;33:169-81. doi: 10.1146/annurev.en.33.010188.001125.
Zotero 插件