Sousa R L, Cardoso T C, Paulillo A C, Montassier H J, Pinto A A
Faculdade de Ciências Agrárias e Veterinérias, Universidade Estadual Paulista, UNESP, Departamento de Microbiologia e Patologia Animal, Jaboticabal-SP, Brasil.
J Zoo Wildl Med. 1999 Sep;30(3):459-61.
Ten young partridges (Rhynchotus rufescens) were vaccinated with the lentogenic strain of Newcastle disease virus. Another eight unvaccinated birds were kept in close contact with the treated flock. Antibodies levels were measured over the course of 3 mo in all birds using the hemagglutination inhibition (HI) test and the liquid-phase blocking enzyme-linked immunosorbent assay (LPB-ELISA). The LPB-ELISA was standardized, and the results were compared with those obtained with the HI test. Antibodies increased after 23 days postvaccination in 16 birds with no side effects as determined by both the HI test and the LPB-ELISA.
十只小鹧鸪(红嘴鹧鸪)用新城疫病毒弱毒株进行了疫苗接种。另外八只未接种疫苗的鸟与经处理的鸟群密切接触。使用血凝抑制(HI)试验和液相阻断酶联免疫吸附测定(LPB-ELISA)在3个月的时间内对所有鸟类的抗体水平进行了测量。LPB-ELISA进行了标准化,并将结果与HI试验获得的结果进行了比较。通过HI试验和LPB-ELISA确定,接种疫苗后23天,16只鸟的抗体增加,且无副作用。