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[间接酶联免疫吸附法与血凝抑制试验检测疫苗接种鸡新城疫抗体的比较]

[Comparison of the indirect enzyme-bound immunosorbent method with the hemagglutination inhibition test in detecting Newcastle disease antibodies in vaccinated chickens].

作者信息

Dumanova L, Chenchev I, Dimitrova E

出版信息

Vet Med Nauki. 1983;20(8):17-23.

PMID:6364558
Abstract

The sensitivity of ELISA and the hemagglutination-inhibition test was comparatively studied in the demonstration of Newcastle disease antibodies in the sera of 57 vaccinated birds. Differences were established in the antibody titers as demonstrated by the two methods. The sensitivity of ELISA was found to be 100 times higher than that of the HI-test. Discussed is the possible use of ELISA in cases where no antibodies or low-titer hemagglutinating ones are demonstrated via the HI-test.

摘要

对57只接种疫苗鸡的血清进行新城疫抗体检测时,对酶联免疫吸附测定(ELISA)和血凝抑制试验的敏感性进行了比较研究。两种方法所显示的抗体效价存在差异。发现ELISA的敏感性比血凝抑制试验高100倍。文中讨论了在通过血凝抑制试验未检测到抗体或血凝抗体效价较低的情况下,ELISA可能的应用。

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