Gloaguen V, Morvan H, Hoffmann L, Plancke Y, Wieruszeski J M, Lippens G, Strecker G
Equipe de Blycobiologie Végétale, Institut de Biotechnologie, Université de Limoges, France.
Eur J Biochem. 1999 Dec;266(3):762-70. doi: 10.1046/j.1432-1327.1999.00879.x.
The capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus has been subjected to a specific degradation with lithium in ethylenediamine. The released undecasaccharide attached to one unit of tetrahydroxycyclopentanecarboxylic acid has been characterized by a combination of 2D nuclear magnetic resonance spectroscopy, mass spectrometry, monosaccharidic composition and linkage analyses. From the overlap of the structure of this oligosaccharide with previously identified di-, tri- and pentasaccharides released by mild acid hydrolysis, the capsular polysaccharide was deduced to have a pentadecasaccharide repeating unit with the following structure:
嗜热蓝细菌层理鞭枝藻产生的荚膜多糖已用锂在乙二胺中进行了特异性降解。通过二维核磁共振光谱、质谱、单糖组成和连接分析相结合的方法对连接一个四羟基环戊烷羧酸单元的释放出的十一糖进行了表征。根据该寡糖的结构与先前通过温和酸水解释放出的二糖、三糖和五糖结构的重叠情况，推断荚膜多糖具有以下结构的十五糖重复单元：
