Gloaguen V, Wieruszeski J M, Strecker G, Hoffmann L, Morvan H
Laboratoire de Biologie Cellulaire Végétale et Valorisation des Espèces Ligneuses, Université de Limoges, France.
Int J Biol Macromol. 1995 Dec;17(6):387-93. doi: 10.1016/0141-8130(96)81851-4.
This study deals with the chemical characterization of a capsular polysaccharide (CPS) produced by a thermal biomass largely comprising the cyanobacterium Mastigocladus laminosus. The sugar moiety of this polymer is composed of seven neutral monosaccharides (Rha, Fuc, Ara, Xyl, Man, Glc, Gal) and two uronic acids (GalA, GlcA). Proteins represent 18% of the dry weight of the CPS. Organic acid substituents (acetate, pyruvate, succinate) were also detected and estimated by high-performance liquid chromatography. The presence of sulfate groups (5% w/w) was observed, which represents a relatively rare feature for cyanobacteria. Acidic hydrolysis of the purified polysaccharide led to the isolation of four oligosaccharidic fractions. NMR spectroscopy studies of two of the four purified oligosaccharides allowed them to be identified as: alpha-GlcA-(1-->2)-alpha-GalA-(1-->2)-Man and alpha-GlcA-(1-->2)-alpha-GalA-(1-->2)-beta-Man-(1-->4)-beta-Gal-(1 -->2)-Rha.
本研究涉及一种由主要包含蓝藻层理鞭枝藻的热生物质产生的荚膜多糖（CPS）的化学表征。该聚合物的糖部分由七种中性单糖（鼠李糖、岩藻糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖）和两种糖醛酸（半乳糖醛酸、葡萄糖醛酸）组成。蛋白质占CPS干重的18%。还通过高效液相色谱法检测并估算了有机酸取代基（乙酸盐、丙酮酸盐、琥珀酸盐）。观察到硫酸基团的存在（5% w/w），这对蓝藻来说是一个相对罕见的特征。纯化多糖的酸水解导致分离出四个寡糖级分。对四个纯化寡糖中的两个进行核磁共振光谱研究，使它们被鉴定为：α-葡萄糖醛酸-（1→2）-α-半乳糖醛酸-（1→2）-甘露糖和α-葡萄糖醛酸-（1→2）-α-半乳糖醛酸-（1→2）-β-甘露糖-（1→4）-β-半乳糖-（1→2）-鼠李糖。
