De Rose R, McKenna R V, Cobon G, Tennent J, Zakrzewski H, Gale K, Wood P R, Scheerlinck J P, Willadsen P
CRC for Vaccine Technology Unit, CSIRO Division of Animal Health, Parkville, Vic., Australia.
Vet Immunol Immunopathol. 1999 Nov 30;71(3-4):151-60. doi: 10.1016/s0165-2427(99)00038-0.
Vaccination of sheep with a plasmid bearing the full length gene for the tick antigen Bm86 either alone or co-administered with plasmid carrying the ovine genes for the cytokines, granulocyte and macrophage colony stimulating factor (GM-CSF) or interleukin (IL)-1beta induced a relatively low level of protection against subsequent tick infestation. This tick damage reached statistical significance only for the groups which were vaccinated with plasmid encoding for Bm86, co-administered with plasmid encoding for ovine GM-CSF. Antibody titres measured against Bm86 were also low in all groups injected with the Bm86 DNA vaccine. Antibody production and anti-tick effect were significantly less than that achieved by two vaccinations with recombinant Bm86 protein. In all cases only a low level of antigen-specific stimulation of peripheral blood lymphocytes was recorded, as measured either by the incorporation of tritiated thymidine or the release of IFN-gamma. Injection of DNA encoding for Bm86, either alone or with co-administered cytokine genes, did however prime for a strong subsequent antibody response following a single injection of recombinant Bm86 protein in adjuvant. Antibody production nevertheless appeared to be slightly less effective than following two vaccinations with recombinant protein. The persistence of antibody following vaccination was the same regardless of the method of primary sensitization. In all cases the half-life of the antibody response was approximately 40-50 days indicating that, in contrast to results reported in mice, DNA vaccination in sheep did not result in sustained antibody production.
用携带蜱抗原Bm86全长基因的质粒单独或与携带绵羊细胞因子、粒细胞和巨噬细胞集落刺激因子（GM-CSF）或白细胞介素（IL）-1β基因的质粒共同给绵羊接种疫苗，对随后的蜱侵袭诱导的保护水平相对较低。仅对于接种编码Bm86的质粒并与编码绵羊GM-CSF的质粒共同接种的组，这种蜱损伤达到统计学显著水平。在所有注射Bm86 DNA疫苗的组中，针对Bm86测量的抗体滴度也很低。抗体产生和抗蜱效果明显低于两次接种重组Bm86蛋白所达到的效果。在所有情况下，通过掺入氚标记的胸腺嘧啶或释放干扰素-γ测量，仅记录到外周血淋巴细胞的低水平抗原特异性刺激。然而，单独注射编码Bm86的DNA或与共同注射的细胞因子基因一起注射，在单次注射佐剂中的重组Bm86蛋白后确实引发了强烈的后续抗体反应。然而，抗体产生似乎比两次接种重组蛋白后的效果略差。无论初次致敏方法如何，接种疫苗后抗体的持久性是相同的。在所有情况下，抗体反应的半衰期约为40-50天，这表明与小鼠中报道的结果相反，绵羊中的DNA疫苗接种并未导致持续的抗体产生。
