Kerr J R, Rigg G P, Matthews R C, Burnie J P
Pertussis Reference Laboratory, Infectious Diseases Research Group, University of Manchester, Oxford Road, Manchester, M13 9WL, U.K.
Microb Pathog. 1999 Dec;27(6):349-67. doi: 10.1006/mpat.1999.0307.
Type III secretory genes(Bscl, J, K, L, N and O) have recently been identified in Bordetella bronchiseptica and shown to be under the control of the BvgAS locus. We examined a 35 616 byte DNA sequence amplified from Bordetella pertussis Tohama I for homology with known type III secretory genes in Yersinia spp. and Pseudomonas sppand a total of 20 homologous open reading frames were detected. Putative type III secretion proteins in B. pertussis were designated according to their homology with type III secretion proteins in B. bronchiseptica, Yersinia and Pseudomonas. These ORFs were arranged in two putative operons, which together we have designated as the BpeI locus. The first spans nucleotides 23385-7888 and encodes the putative proteins LcrH1, BopD, BopB, LcfH2, BscI, BscJ, BscK, BscL, BscN, BscO, BscQ, BscR, BscS, BscT, BscU, and BscC, in this order. The second spans nucleotides 23580-29863 and encodes the putative proteins LcrE, LcrD, BscD and BscF, in this order. The homology of these proteins to type III secretory proteins was B. bronchiseptica (73-99%), Yersinia spp. (17-65%), Pseudomonas spp. (18-64%). The B. pertussis proteins were similar to their homologues in B. bronchiseptica, Yersinia and Pseudomonas in terms of length, molecular weight and isoelectric point. Coiled-coil domains were detected in putative translocation proteins, BopB and BopD. BopB and BopD were similar to each other, to the RTX toxin family and to cyaA, cyaB, cyaD and cyaE. The percentage G+C content of the sequence analysed was 66.16%, which is similar to the published percentage G+C (67-70%) for the B. pertussis chromosome.
III型分泌基因（Bscl、J、K、L、N和O）最近在支气管败血博德特氏菌中被鉴定出来，并显示受BvgAS位点的控制。我们检测了从百日咳博德特氏菌Tohama I扩增的一段35616字节的DNA序列，以寻找与耶尔森氏菌属和假单胞菌属中已知III型分泌基因的同源性，共检测到20个同源开放阅读框。百日咳博德特氏菌中的推定III型分泌蛋白根据它们与支气管败血博德特氏菌、耶尔森氏菌和假单胞菌中III型分泌蛋白的同源性来命名。这些开放阅读框排列在两个推定的操纵子中，我们将其统称为BpeI位点。第一个操纵子跨度为核苷酸23385 - 7888，依次编码推定蛋白LcrH1、BopD、BopB、LcfH2、BscI、BscJ、BscK、BscL、BscN、BscO、BscQ、BscR、BscS、BscT、BscU和BscC。第二个操纵子跨度为核苷酸23580 - 29863，依次编码推定蛋白LcrE、LcrD、BscD和BscF。这些蛋白与III型分泌蛋白的同源性在支气管败血博德特氏菌中为73 - 99%，在耶尔森氏菌属中为17 - 65%，在假单胞菌属中为18 - 64%。百日咳博德特氏菌的蛋白在长度、分子量和等电点方面与其在支气管败血博德特氏菌、耶尔森氏菌和假单胞菌中的同源物相似。在推定的转运蛋白BopB和BopD中检测到卷曲螺旋结构域。BopB和BopD彼此相似，与RTX毒素家族以及cyaA、cyaB、cyaD和cyaE相似。所分析序列的G + C含量百分比为66.16%，与已发表的百日咳博德特氏菌染色体的G + C百分比（67 - 70%）相似。
