细胞间相互作用：衣藻配子接触过程中糖基转移酶外切酶系统的增强。
Cell-cell interactions: enhancement of glycosyl transferase ectoenzyme systems during Chlamydomonas gametic contact.
作者信息
McLean R J, Bosmann H B
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):310-3. doi: 10.1073/pnas.72.1.310.
Glycosyl transferase ectoenzyme systems that transfer galactose, glucose, N-acetylglucosamine, N-acetylneuraminic acid, mannose, and fucose have been detected on vegetative cells and gametes of Chlamydomonas moewusii. Gametes have higher levels of activity of the transferase ectoenzyme systems than morphologically identical vegetative cells, as determined by transfer of monosaccharide onto endogenous cell surface acceptors. When (plus) and (minus) gametes are mixed, there is a significant increase in the activity of transferase ectoenzyme systems. No enhancement in activity of transferase ectoenzyme systems occurs when (plus) and (minus) vegetative cells are mixed. Flagellar membrane vesicles obtained from (plus) and (minus) gametes show high activity of transferase ectoenzyme systems per mg of protein and also demonstrate enhanced activity upon mixing. Therefore, glycosyl transferases and acceptors seem to be located on the flagellar membrane and appear to have a function particularly related to gametic cells. The mechanism of cellular adhesion or recognition proposed by Roseman (1970, Chem. Phys. Lipids 5, 270-297), involving glycosyl transferases and acceptors, is strongly suggested by our data for the mating reaction in Chlamydomonas.
在莱茵衣藻的营养细胞和配子上已检测到能转移半乳糖、葡萄糖、N-乙酰葡糖胺、N-乙酰神经氨酸、甘露糖和岩藻糖的糖基转移酶胞外酶系统。通过将单糖转移到内源性细胞表面受体上测定，配子的转移酶胞外酶系统活性水平高于形态相同的营养细胞。当（正）配子和（负）配子混合时，转移酶胞外酶系统的活性显著增加。当（正）营养细胞和（负）营养细胞混合时，转移酶胞外酶系统的活性没有增强。从（正）配子和（负）配子获得的鞭毛膜囊泡每毫克蛋白质显示出高转移酶胞外酶系统活性，并且在混合时也表现出增强的活性。因此，糖基转移酶和受体似乎位于鞭毛膜上，并且似乎具有与配子细胞特别相关的功能。我们关于莱茵衣藻交配反应的数据强烈表明了罗斯曼（1970年，《化学与物理脂质》5，270 - 297）提出的涉及糖基转移酶和受体的细胞黏附或识别机制。
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