Gottesfeld J M, Murphy R F, Bonner J
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4404-8. doi: 10.1073/pnas.72.11.4404.
Rat-liver chromatin has bee fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of nuclease-resistant complexes in these fractions has been investigated. About half of the DNA of both fractions is resistant to attack by tne endonuclease DNase II. The nuclease-resistant structures of inactive chromatin are DNA-histone complexes (v-bodies) which sediment at 11-13 S. Template-active chromatin yields two peaks of nuclease-resistant nucleoprotein. These complexes sediment at 14 and 19 S, and contain DNA, RNA, histone, and nonhistone chromosomal proteins. Polyacrylamide gel electrophoresis reveals a complex pattern of chromatin proteins, suggesting that the complexes are heterogeneous in composition.
大鼠肝脏染色质已被分离为转录活性区和非活性区[戈特斯费尔德等人(1974年),《美国国家科学院院刊》71卷,2193 - 2197页],并对这些组分中核酸酶抗性复合物的分布进行了研究。两个组分中约一半的DNA对核酸内切酶DNase II的攻击具有抗性。非活性染色质的核酸酶抗性结构是在11 - 13 S沉降的DNA - 组蛋白复合物(v体)。模板活性染色质产生两个核酸酶抗性核蛋白峰。这些复合物在14和19 S沉降,并且包含DNA、RNA、组蛋白和非组蛋白染色体蛋白。聚丙烯酰胺凝胶电泳揭示了染色质蛋白的复杂模式,表明这些复合物在组成上是异质的。