Chasin M, Scott C, Shaw C, Persico F
Int Arch Allergy Appl Immunol. 1979;58(1):1-10. doi: 10.1159/000232167.
In this report, we describe a new in vitro experimental system for monitoring the release, and inhibition of release, of [3H]-serotonin from rat peritoneal mast cells, which can be used as a novel screen for evaluation of compounds effective in immediate hypersensitivity reactions. This assay is an improvement on existing assays since the cells are not required to be exposed to hormones at any time during the procedure. In this assay, 1 microgram/ml of compound 48/80 consistently produced a 6- to 8-fold-increase in release of serotonin with a low background release of 4-5%. Disodium chromoglycate (DSCG), a standard inhibitor of histamine release, effectively inhibited compound-48/80-stimulated serotonin release with an average I50 of 2.1 X 10(-4) M. Several other potential antiasthmatic agents were far more potent than DSCG.
在本报告中，我们描述了一种新的体外实验系统，用于监测大鼠腹膜肥大细胞中[3H] - 血清素的释放及释放抑制情况，该系统可作为一种新型筛选方法，用于评估对速发型超敏反应有效的化合物。此检测方法是对现有检测方法的改进，因为在整个实验过程中细胞无需暴露于激素。在该检测中，1微克/毫升的化合物48/80能持续使血清素释放增加6至8倍，背景释放率低至4 - 5%。色甘酸二钠（DSCG）是组胺释放的标准抑制剂，能有效抑制化合物48/80刺激的血清素释放，平均半数抑制浓度（I50）为2.1×10^(-4) M。其他几种潜在的抗哮喘药物比DSCG的效力要强得多。
