Yamagishi J, Hu Y, Zheng J, Bando H
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Arch Virol. 1999;144(11):2111-24. doi: 10.1007/s007050050626.
We determined the complete nucleotide sequence of an infectious clone of the cockroach small spherical virus (CSSV) genome. Analysis of the genome organization and the predicted viral protein sequences showed clearly that this virus should be classified as a new member of the subfamily Densovirinae, genus Densovirus, and should be designated as PfDNV. However, our data revealed some differences between the gene expression strategies used by PfDNV and other DNVs. An internal promoter, in addition to the promoter (p3) at the genome terminus, was observed at map unit 18 (p18), implying transcriptional regulation of generation of the nonstructural proteins of PfDNV. Furthermore, the structural analysis of cDNAs complementary to mRNAs from the region coding for structural proteins suggested alternative splicing and polyadenylation as means for generation of the structural proteins of PfDNV.
我们测定了蟑螂小球形病毒（CSSV）基因组感染性克隆的完整核苷酸序列。对基因组结构和预测的病毒蛋白序列的分析清楚地表明，这种病毒应归类为浓核病毒亚科浓病毒属的一个新成员，并应命名为PfDNV。然而，我们的数据揭示了PfDNV与其他浓病毒所采用的基因表达策略之间存在一些差异。除了基因组末端的启动子（p3）外，在图谱单位18（p18）处还观察到一个内部启动子，这意味着PfDNV非结构蛋白生成的转录调控。此外，对编码结构蛋白区域的mRNA互补cDNA的结构分析表明，可变剪接和多聚腺苷酸化是PfDNV结构蛋白生成的方式。
