Hu Y, Zheng J, Iizuka T, Bando H
Department of Virology, Wuhan University, China.
Arch Virol. 1994;138(3-4):365-72. doi: 10.1007/BF01379139.
We purified a causing agent of fetal disease for smoky-brown cockroach Periplaneta fuliginosa, which was designated as "cockroach small spherical virus (CSSV)". Purified virus particles had a diameter of 22 +/- 0.6 nm and contained DNA as a single-stranded form. However, the extraction of DNA under condition of appropriate high salt and elevated temperature yielded a double-stranded DNA with a size of 5,500 nucleotides. These results were quite similar to those of other densoviruses (DNVs). The CSSV had five structural proteins (VP1: 52 KDa, VP2: 56 KDa, VP3: 79 KDa, VP4: 82 KDa, and VP5: 105 KDa). The SDS-PAGE profile of these proteins was quite different from that of the cockroach DNV previously reported and was rather similar to that of Bombyx mori (Bm) DNV-1. An immunochemical study, however, demonstrated that there was no immunological relationship between the CSSV and the Bm DNV-1. These data suggest that the CSSV is a new member of DNV.
我们纯化了致烟褐色蟑螂（Periplaneta fuliginosa）胎儿疾病的病原体，将其命名为“蟑螂小球形病毒（CSSV）”。纯化的病毒颗粒直径为22±0.6纳米，含有单链形式的DNA。然而，在适当的高盐和高温条件下提取DNA，得到了大小为5500个核苷酸的双链DNA。这些结果与其他浓核病毒（DNV）的结果非常相似。CSSV有五种结构蛋白（VP1：52千道尔顿，VP2：56千道尔顿，VP3：79千道尔顿，VP4：82千道尔顿，VP5：105千道尔顿）。这些蛋白质的SDS-PAGE图谱与先前报道的蟑螂DNV有很大不同，与家蚕（Bm）DNV-1的图谱相当相似。然而，免疫化学研究表明，CSSV与Bm DNV-1之间没有免疫关系。这些数据表明CSSV是DNV的一个新成员。
