Lin K C
Department of Obstetrics and Gynecology, Kaohsiung Medical University, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1999 Oct;15(10):597-602.
A possible role of ovarian steroids on the modulation of leptin production has recently been suggested. To investigate the relationship between leptin and ovarian steroids during the normal menstrual cycle, twenty-three healthy, normal-weight women were enrolled in the study. Blood was collected into chilled EDTA tubes on cycle days (D) 3, 7, 10, preovulatory (D13-15), midluteal (D 21-23) and late luteal (D 25-27) phases. Plasma leptin, estradiol (E2), progesterone (P), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured by RIA. Leptin levels showed no significant changes throughout the follicular (D7 and D10), preovulatory (8.6 +/- 0.5 ng/mL), midluteal (8.9 +/- 0.6 ng/mL) and late luteal (7.7 +/- 0.4 ng/mL) phases, as compared with those of D3 (7.4 +/- 0.5 ng/mL) level. There was no positive correlation between leptin and E2 levels in D 3 and D 7 (r = 0.14, P > 0.05) or in D 10 and preovulatory day (r = 0.25, P > 0.05). Also, no positive correlation between leptin and E2 (r = 0.12, P > 0.05) or leptin and P (r = 0.17, P > 0.05) levels were observed in the mid- and late luteal phases. These data clearly indicate that physiological alterations of circulating E2 and P concentrations have no significant influence on the leptin changes during normal menstrual cycle.
最近有人提出卵巢类固醇在调节瘦素产生方面可能发挥作用。为了研究正常月经周期中瘦素与卵巢类固醇之间的关系，23名健康、体重正常的女性参与了该研究。在月经周期的第3天、第7天、第10天、排卵前期（第13 - 15天）、黄体中期（第21 - 23天）和黄体后期（第25 - 27天），将血液采集到预冷的乙二胺四乙酸（EDTA）管中。采用放射免疫分析法（RIA）测定血浆瘦素、雌二醇（E2）、孕酮（P）、黄体生成素（LH）和卵泡刺激素（FSH）。与第3天（7.4±0.5 ng/mL）的水平相比，瘦素水平在整个卵泡期（第7天和第10天）、排卵前期（8.6±0.5 ng/mL）、黄体中期（8.9±0.6 ng/mL）和黄体后期（7.7±0.4 ng/mL）均无显著变化。在第3天和第7天（r = 0.14，P>0.05）或第10天与排卵前期（r = 0.25，P>0.05），瘦素与E2水平之间无正相关。此外，在黄体中期和后期，也未观察到瘦素与E2（r = 0.12，P>0.05）或瘦素与P（r = 0.17，P>0.05）水平之间存在正相关。这些数据清楚地表明，在正常月经周期中，循环E2和P浓度的生理变化对瘦素变化没有显著影响。
