McCarron B, Fox R, Wilson K, Cameron S, McMenamin J, McGregor G, Pithie A, Goldberg D
Department of Infectious Diseases and Tropical Medicine, Brownlee Centre, Gartnavel General Hospital, Glasgow, UK.
J Viral Hepat. 1999 Nov;6(6):453-6. doi: 10.1046/j.1365-2893.1999.00197.x.
In the UK, there have been few studies of the seroprevalence of antibodies to hepatitis C virus (anti-HCV). As part of an ongoing prevalence study of HCV in injecting drug users, we have developed a technique for detecting anti-HCV in blood spots dried on filter paper using a commercially available assay. Subjects with and without serum anti-HCV were studied. The manufacturer's recommended cut-off (CO) for a positive anti-HCV result is kit-dependent, and therefore a ratio of test result (T) to kit CO was used to standardize results. T/CO values greater than 0.99 had a sensitivity of 100% and a specificity of 87.5% for anti-HCV detection. T/CO values greater than 1.99 had a sensitivity of 97.2% and a specificity of 100%. Hence, testing dried blood spots may be useful for detecting anti-HCV in epidemiological studies and as a diagnostic test in patients with poor peripheral venous access.
在英国，针对丙型肝炎病毒抗体（抗-HCV）血清流行率的研究较少。作为正在进行的注射吸毒者中HCV流行率研究的一部分，我们开发了一种使用市售检测方法在滤纸上干燥血斑中检测抗-HCV的技术。对有和没有血清抗-HCV的受试者进行了研究。抗-HCV检测结果呈阳性时，制造商推荐的临界值（CO）因试剂盒而异，因此使用检测结果（T）与试剂盒CO的比值来标准化结果。T/CO值大于0.99时，抗-HCV检测的灵敏度为100%，特异性为87.5%。T/CO值大于1.99时，灵敏度为97.2%，特异性为100%。因此，检测干燥血斑可能有助于在流行病学研究中检测抗-HCV，并作为外周静脉通路较差患者的诊断测试。
