Villar Livia Melo, Cruz Helena Medina, Deodato Raissa Martins, Miguel Juliana Custódio, da Silva Elisangela Ferreira, Flores Geane Lopes, Lewis-Ximenez Lia Laura
Viral Hepatitis Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Hélio and Peggy Pereira Pavilion - Ground Floor - Office B09, FIOCRUZ Av. Brasil, 4365 - Manguinhos, Rio de Janeiro, RJ, 210360-040, Brazil.
BMC Res Notes. 2019 Aug 20;12(1):523. doi: 10.1186/s13104-019-4547-y.
Dried blood spots (DBSs) can be used as an alternative to serum samples because they are easily collected and can be transported without refrigeration to reference laboratories for diagnosis. The present study was performed to evaluate the utility of electrochemiluminescence immunoassay "ECLIA" for anti-HCV, HBsAg and anti-HBc detection from DBS samples.
Anti-HCV was detected in 103 DBS samples from 108 paired, positive serum and undetected in 364 DBS samples from 366 paired, negative specimens, giving a sensitivity of 95.4% and a specificity of 99.4%. HBsAg was detected in 67 DBS samples out of 71 positive, paired serum and was undetected among 295 DBS samples from 298 paired, negative specimens, giving a sensitivity and specificity of 94.4% and 99%, respectively. Anti-HBc was detected in 160 DBS samples from 185 paired, positive serum specimens and undetected in 349 DBS samples from 357 paired, negative serum specimens, giving a sensitivity of 86.5% and a specificity of 97.8%. Overall, the Kappa index indicated a high agreement between results obtained for the serum and DBS samples (k: 0.95, 0.93 and 0.86 for anti-HCV, HBsAg, anti-HBc, respectively). In conclusion, the ECLIA test could be used for detecting hepatitis B and C markers in DBS.
干血斑（DBS）可作为血清样本的替代物，因为其采集方便，无需冷藏即可运输至参考实验室进行诊断。本研究旨在评估电化学发光免疫分析法（ECLIA）用于检测DBS样本中抗丙型肝炎病毒（抗-HCV）、乙型肝炎表面抗原（HBsAg）和抗乙型肝炎核心抗体（抗-HBc）的效用。
在108对配对的阳性血清中的103份DBS样本中检测到抗-HCV，在366对配对的阴性样本中的364份DBS样本中未检测到，灵敏度为95.4%，特异性为99.4%。在71份阳性配对血清中的67份DBS样本中检测到HBsAg，在298对配对的阴性样本中的295份DBS样本中未检测到，灵敏度和特异性分别为94.4%和99%。在185对配对的阳性血清样本中的160份DBS样本中检测到抗-HBc，在357对配对的阴性血清样本中的349份DBS样本中未检测到，灵敏度为86.5%，特异性为97.8%。总体而言，卡帕指数表明血清和DBS样本的检测结果之间具有高度一致性（抗-HCV、HBsAg、抗-HBc的k值分别为0.95、0.93和0.86）。总之，ECLIA检测可用于检测DBS中的乙型和丙型肝炎标志物。
