Seo K, Akiyoshi H, Ohnishi Y
Department of Biological Chemistry, Faculty of Integrated Arts and Sciences, School of Medicine, The University of Tokushima, Japan.
Microbiol Immunol. 1999;43(11):1017-25. doi: 10.1111/j.1348-0421.1999.tb01231.x.
An amphotericin B (AmB)-resistant mutant was isolated from a wild-type AmB-susceptible strain of Aspergillus flavus by serial transfer of conidia on agar plates containing stepwise increased concentrations of AmB up to 100 microg ml-1. The acquired resistance of mycelia was specific for polyene-antibiotics AmB, nystatin and trichomycin. Spheroplasts derived from the resistant mycelia were as susceptible to AmB as the wild-type. Chemical analysis of the cell wall revealed that levels of alkali-soluble and -insoluble glucans were significantly higher in the resistant mycelia as compared to those in the wild-type. When resistant mycelia were treated with SDS, they adsorbed as much AmB as wild-type mycelia. These results suggest that alterations in the cell wall components of mycelia, especially 1,3-alpha-glucan and protein complex in the outermost wall layer, lead to AmB resistance in A. flavus.
通过在含有逐步增加浓度直至100微克/毫升两性霉素B（AmB）的琼脂平板上连续转接分生孢子，从黄曲霉的野生型AmB敏感菌株中分离出一株AmB抗性突变体。菌丝体获得的抗性对多烯抗生素AmB、制霉菌素和曲古霉素具有特异性。源自抗性菌丝体的原生质体对AmB的敏感性与野生型相同。细胞壁的化学分析表明，与野生型相比，抗性菌丝体中碱溶性和碱不溶性葡聚糖的水平显著更高。当用十二烷基硫酸钠（SDS）处理抗性菌丝体时，它们吸附的AmB与野生型菌丝体一样多。这些结果表明，菌丝体细胞壁成分的改变，尤其是最外层壁层中的1,3-α-葡聚糖和蛋白质复合物，导致黄曲霉对AmB产生抗性。
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