Ramanandraibe E, Younsi M, Coulon J, Loppinet V, Hakkou A, Bonaly R
Université Henri Poincaré, Faculté de Pharmacie, Laboratoire de Biochimie microbienne, Nancy, France.
Res Microbiol. 1998 Feb;149(2):109-18. doi: 10.1016/s0923-2508(98)80026-0.
In Kluyveromyces lactis, the cell wall compositions of Kl (ATCC 96897), a wild sensitive strain, and Klm (ATCC 96896), a strain resistant to amphotericin B (AmB), were shown to be very different, since the walls in the latter were significantly enriched in hexosamine, but had a reduced content in phosphate and amino acid. In both strains, the cell walls limited their sensitivity to this antifungal agent. The absence of cell wall increased the sensitivity of the cells to this polyene by 5 to 10-fold. When the cells were treated with enzymes such as pronase and chitinase in order to change the cell wall structure just before inoculation, the yeasts appeared more resistant to the antibiotic. However, treatments with chymopapain and phospholipase C did not significantly change the sensitivity of the two strains to this agent. Cells treated with acid phosphatase displayed a longer lag phase than the control cells. In addition, when cultured in the presence of AmB, the cells were less sensitive to this agent. The present results reveal that both a change in the ionic charges of the cell wall and an alteration in the cell wall structure modified the sensitivity of these yeast strains to AmB.
在乳酸克鲁维酵母中，野生敏感菌株Kl（ATCC 96897）和对两性霉素B（AmB）耐药的菌株Klm（ATCC 96896）的细胞壁组成显示出非常不同，因为后者的细胞壁中氨基己糖显著富集，但磷酸盐和氨基酸含量降低。在这两种菌株中，细胞壁限制了它们对这种抗真菌剂的敏感性。细胞壁的缺失使细胞对这种多烯的敏感性提高了5至10倍。当在接种前用链霉蛋白酶和几丁质酶等酶处理细胞以改变细胞壁结构时，酵母对该抗生素的抗性增强。然而，用木瓜凝乳蛋白酶和磷脂酶C处理并没有显著改变这两种菌株对该药物的敏感性。用酸性磷酸酶处理的细胞比对照细胞表现出更长的延迟期。此外，当在AmB存在下培养时，细胞对该药物的敏感性降低。目前的结果表明，细胞壁离子电荷的变化和细胞壁结构的改变都会改变这些酵母菌株对AmB的敏感性。
