Maser J, Osanna A, Wang Y, Jacobsen C, Kirz J, Spector S, Winn B, Tennant D
Department of Physics and Astronomy, State University of New York at Stony Brook, Stony Brook, New York 11794-3800, USA.
J Microsc. 2000 Jan;197(Pt 1):68-79. doi: 10.1046/j.1365-2818.2000.00630.x.
We have developed a cryo scanning transmission X-ray microscope which uses soft X-rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 microm at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II. Tomography. J. Microsc. 197, 80-93) or spectroscopic analysis.
我们研发了一种低温扫描透射X射线显微镜，它利用国家同步辐射光源产生的软X射线。该系统能够在约100K的温度下对厚度达10微米的冷冻水合标本进行成像。我们展示了冷冻水合真核细胞的图像和光谱，并证明生物标本在高达约10¹⁰戈瑞的辐射剂量下不会出现质量损失或形态变化。这使得诸如断层扫描（Wang等人，（2000年）《使用低温扫描透射X射线显微镜的软X射线显微镜学：II. 断层扫描》。《显微镜学杂志》197卷，第80 - 93页）或光谱分析等需要对同一样本区域进行多张图像拍摄的研究成为可能。
