Noyan S, Kahveci Z, Cavusoglu I, Minbay F Z, Sunay F B, Sirmali S A
Department of Histology and Embryology, Faculty of Medicine, Uludag University, 16384 Bursa, Turkey.
J Microsc. 2000 Jan;197(Pt 1):101-6. doi: 10.1046/j.1365-2818.2000.00609.x.
Modified gold impregnation is one of the methods that are used in light microscopical demonstration of hepatic perisinusoidal cells. This method has some disadvantages, such as restriction of fixation time to 16 h, which allows limited time for processing the tissues, especially when dealing with a large amount of material, and a long impregnation time (16-24 h). We investigated the effect of prolonged fixation on the staining of sections, to shorten the time needed for gold impregnation by using microwave irradiation. Liver specimens were fixed in Baker's calcium-formalin for different periods of time. After fixation, frozen sections were impregnated in gold chloride solution either at room temperature or in a microwave oven. The staining quality of the sections which had been impregnated in the microwave oven for a much shorter time were equal to or even superior to the ones impregnated at room temperature. Prolonging the fixation time up to 7 days did not affect the staining results by microwave irradiation, whereas satisfactory results were not obtained from sections stained at room temperature and fixed for more than 3 days. We conclude that microwave irradiation can be used to shorten the impregnation time in gold chloride solution and the duration of fixation can be prolonged up to 3 days in the original method and up to 7 days when microwave irradiation is used during impregnation.
改良金浸染法是用于光镜下显示肝血窦周细胞的方法之一。该方法存在一些缺点，比如固定时间限制在16小时，这使得处理组织的时间有限，尤其是在处理大量材料时，而且浸染时间长（16 - 24小时）。我们研究了延长固定时间对切片染色的影响，以通过使用微波辐射来缩短金浸染所需的时间。将肝脏标本用贝克氏钙甲醛固定不同时间。固定后，冰冻切片在室温或微波炉中用氯化金溶液浸染。在微波炉中浸染时间短得多的切片的染色质量与在室温下浸染的切片相当甚至更好。将固定时间延长至7天不会影响微波辐射的染色结果，而在室温下染色且固定超过3天的切片则未获得满意结果。我们得出结论，微波辐射可用于缩短在氯化金溶液中的浸染时间，在原方法中固定时间可延长至3天，在浸染过程中使用微波辐射时可延长至7天。
