使用扫描离子电导显微镜进行细胞体积测量。
Cell volume measurement using scanning ion conductance microscopy.
作者信息
Korchev Y E, Gorelik J, Lab M J, Sviderskaya E V, Johnston C L, Coombes C R, Vodyanoy I, Edwards C R
机构信息
Division of Medicine, Imperial College School of Medicine, Medical Research Council Clinical Sciences Centre, Hammersmith Campus, Du Cane Road, London W12 0NN, United Kingdom.
出版信息
Biophys J. 2000 Jan;78(1):451-7. doi: 10.1016/S0006-3495(00)76607-0.
We report a novel scanning ion conductance microscopy (SICM) technique for assessing the volume of living cells, which allows quantitative, high-resolution characterization of dynamic changes in cell volume while retaining the cell functionality. The technique can measure a wide range of volumes from 10(-19) to 10(-9) liter. The cell volume, as well as the volume of small cellular structures such as lamelopodia, dendrites, processes, or microvilli, can be measured with the 2.5 x 10(-20) liter resolution. The sample does not require any preliminary preparation before cell volume measurement. Both cell volume and surface characteristics can be simultaneously and continuously assessed during relatively long experiments. The SICM method can also be used for rapid estimation of the changes in cell volume. These are important when monitoring the cell responses to different physiological stimuli.
我们报告了一种用于评估活细胞体积的新型扫描离子电导显微镜（SICM）技术，该技术能够对细胞体积的动态变化进行定量、高分辨率表征，同时保持细胞功能。该技术可测量从10^(-19)到10^(-9)升的广泛体积范围。细胞体积以及诸如片状伪足、树突、突起或微绒毛等小细胞结构的体积，能够以2.5×10^(-20)升的分辨率进行测量。在测量细胞体积之前，样品无需任何预处理。在相对较长的实验过程中，可以同时且连续地评估细胞体积和表面特征。SICM方法还可用于快速估计细胞体积的变化。在监测细胞对不同生理刺激的反应时，这些都很重要。
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