Kline B J, Lele S S, Lenart P J, Beckman E J, Russell A J
Department of Chemical and Petroleum Engineering and Center for Biotechnology and Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA. ajrche+@pitt.edu
Biotechnol Bioeng. 2000 Feb 20;67(4):424-34.
Despite favorable thermodynamics, high-molecular weight and low-dispersity polyesters are difficult to synthesize biocatalytically in organic solvents. We have reported previously that the elimination of solvent can improve the kinetics and apparent equilibrium significantly (Chaudhary et al., 1997a). We now present the design and use of a batch-stirred enzyme reactor to control the biocatalytic polymerization. Using the reactor, polyester having a molecular weight of 23,400 Da and a polydispersity of 1.69 was synthesized in only 1 h at 60 degrees C. Additional factors like enzyme-deactivation kinetics, enzyme specificity, and initial exothermicity were investigated to develop a better understanding of this complex reaction system.
尽管从热力学角度来看条件有利，但在有机溶剂中通过生物催化合成高分子量且低分散度的聚酯却很困难。我们之前曾报道，去除溶剂可显著改善动力学和表观平衡（乔杜里等人，1997a）。我们现在介绍一种间歇搅拌式酶反应器的设计与应用，以控制生物催化聚合反应。使用该反应器，在60摄氏度下仅1小时就合成了分子量为23400道尔顿、多分散度为1.69的聚酯。还研究了诸如酶失活动力学、酶特异性和初始放热等其他因素，以便更好地理解这个复杂的反应体系。
