一种使用十八烷基硅烷化硅胶从组织中提取某些肽的快速方法。
A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues.
作者信息
Bennett H P, Hudson A M, Kelly L, McMartin C, Purdon G E
出版信息
Biochem J. 1978 Dec 1;175(3):1139-41. doi: 10.1042/bj1751139.
Abstract
Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.
摘要
肽可通过在精心挑选的提取介质中匀浆从组织中回收，随后将上清液吸附在十八烷基硅烷硅胶小柱上。添加到多种组织中的促肾上腺皮质激素和生长抑素的回收率是定量的，并且通过高压液相色谱法测定，这些肽未受损。
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