从大鼠垂体中分离和鉴定阿片样肽：β-促脂素。
Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.
作者信息
Rubinstein M, Stein S, Gerber L D, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052-5. doi: 10.1073/pnas.74.7.3052.
Abstract
Rat beta-lipotropin was isolated from 40 anterior pituitaries using high efficiency chromatography columns and sensitive fluorometric methods. The beta-lipotropin was characterized as to molecular weight, amino acid composition, and generation of opioid activity by trypsinization. Other opioid peptides, as well as a precursor to opioid peptides with a molecular weight larger than that of beta-lipotropin, were observed in this tissue.
摘要
利用高效色谱柱和灵敏的荧光测定法，从40个大鼠垂体前叶中分离出大鼠β-促脂解素。对β-促脂解素的分子量、氨基酸组成以及经胰蛋白酶消化产生阿片样活性进行了表征。在该组织中还观察到了其他阿片肽以及分子量大于β-促脂解素的阿片肽前体。
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