培养条件下分离的两栖类神经元动作电位机制的研究进展
The development of the action potential mechanism of amphibian neurons isolated in culture.
作者信息
Spitzer N C, Lamborghini J E
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1641-5. doi: 10.1073/pnas.73.5.1641.
Abstract
Nerve and muscle cells differentiated morphologically, in cultures of dissociated cells prepared from amphibian neural plate and underlying mesoderm (Xenopus laevis, Nieuwkoop and Faber stage 15). Cultures were grown in a defined medium containing sterile Steinberg's salt solution and 0.1% bovine serum albumin, and maintained for periods up to 5 days.
摘要
在从两栖类神经板和下方中胚层（非洲爪蟾，Nieuwkoop和Faber第15期）制备的解离细胞培养物中，神经细胞和肌肉细胞发生了形态分化。培养物在含有无菌斯坦伯格盐溶液和0.1%牛血清白蛋白的限定培养基中生长，并维持长达5天。
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