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培养条件下分离的两栖类神经元动作电位机制的研究进展

The development of the action potential mechanism of amphibian neurons isolated in culture.

作者信息

Spitzer N C, Lamborghini J E

出版信息

Proc Natl Acad Sci U S A. 1976 May;73(5):1641-5. doi: 10.1073/pnas.73.5.1641.

DOI:10.1073/pnas.73.5.1641
PMID:1064036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430355/
Abstract

Nerve and muscle cells differentiated morphologically, in cultures of dissociated cells prepared from amphibian neural plate and underlying mesoderm (Xenopus laevis, Nieuwkoop and Faber stage 15). Cultures were grown in a defined medium containing sterile Steinberg's salt solution and 0.1% bovine serum albumin, and maintained for periods up to 5 days.

摘要

在从两栖类神经板和下方中胚层（非洲爪蟾，Nieuwkoop和Faber第15期）制备的解离细胞培养物中，神经细胞和肌肉细胞发生了形态分化。培养物在含有无菌斯坦伯格盐溶液和0.1%牛血清白蛋白的限定培养基中生长，并维持长达5天。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/3905a6dc4d4f/pnas00035-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/9d0d10ede6ad/pnas00035-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/7e02cc7a6370/pnas00035-0272-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/3bc42e89f10f/pnas00035-0272-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/3905a6dc4d4f/pnas00035-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/9d0d10ede6ad/pnas00035-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/7e02cc7a6370/pnas00035-0272-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/3bc42e89f10f/pnas00035-0272-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9d5/430355/3905a6dc4d4f/pnas00035-0273-a.jpg

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本文引用的文献

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The culture of small aggregates of amphibian embryonic cells in vitro.两栖类胚胎细胞小聚集体的体外培养。
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The development of the primary sensory system in Xenopus laevis (Daudin).非洲爪蟾（达丁）初级感觉系统的发育
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Surface density of calcium ions and calcium spikes in the barnacle muscle fiber membrane.藤壶肌纤维膜中钙离子的表面密度和钙尖峰
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Region-specific regulation of voltage-gated intrinsic currents in the developing optic tectum of the Xenopus tadpole.非洲爪蟾蝌蚪发育中的视顶盖中电压门控内在电流的区域特异性调节。
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Sequential acquisition of cacophony calcium currents, sodium channels and voltage-dependent potassium currents affects spike shape and dendrite growth during postembryonic maturation of an identified Drosophila motoneuron.在一个已鉴定的果蝇运动神经元胚胎后成熟过程中，刺耳钙电流、钠通道和电压依赖性钾电流的顺序获得会影响动作电位形状和树突生长。
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Simultaneous pre- and post-synaptic electrophysiological recording from Xenopus nerve-muscle co-cultures.对非洲爪蟾神经-肌肉共培养物进行突触前和突触后同时电生理记录。
J Vis Exp. 2013 Mar 11(73):e50253. doi: 10.3791/50253.
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Neural Explant Cultures from Xenopus laevis.非洲爪蟾的神经外植体培养物
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Dissection, culture, and analysis of Xenopus laevis embryonic retinal tissue.非洲爪蟾胚胎视网膜组织的解剖、培养及分析
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Stem Cells. 2012 Sep;30(9):2020-31. doi: 10.1002/stem.1175.
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Activity-dependent neurotransmitter respecification.活动依赖性神经递质再指定。
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J Cell Biol. 1973 Jan;56(1):255-8.
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J Physiol. 1976 Feb;255(1):105-35. doi: 10.1113/jphysiol.1976.sp011272.