Jensen S L, Kühl C, Nielsen O V, Holst J J
Scand J Gastroenterol Suppl. 1976;37:57-61.
The isolation of a porcine pancreas preparation without the attached duodenum is described. The preparation is suitable for study of the secretion of insulin and pancreatic glucagon. The surgical procedure by which the pancreas is removed from tha animal is decribed as well as the perfusion system. The effect of various concentrations of glucose (63, 90, 135 and 180 mg per 100 ml) in the perfusion medium on the secretion of insulin and pancreatic glucagon was investigated in order to observe the performance characteristics of this preparation. Increasing concentrations of glucose caused a significantly greater insulin release, whereas the glucagon response was significantly reduced.
本文描述了一种分离出不带附着十二指肠的猪胰腺制剂的方法。该制剂适用于研究胰岛素和胰高血糖素的分泌。文中描述了从动物体内取出胰腺的手术过程以及灌注系统。为了观察该制剂的性能特征，研究了灌注培养基中不同浓度葡萄糖（每100毫升63、90、135和180毫克）对胰岛素和胰高血糖素分泌的影响。葡萄糖浓度的增加导致胰岛素释放显著增加，而胰高血糖素的反应则显著降低。
