Schwartz T W, Holst J J, Fahrenkrug J, Jensen S L, Nielsen O V, Rehfeld J F, de Muckadell O B, Stadil F
J Clin Invest. 1978 Mar;61(3):781-9. doi: 10.1172/JCI108992.
THE EFFECT OF EFFERENT, PARASYMPATHETIC STIMULATION UPON PANCREATIC POLYPEPTIDE (PP) SECRETION WAS STUDIED IN THREE WAYS: (a) Plasma PP concentrations increased in response to insulin-induced hypoglycemia in both normal subjects, from 11 pM (9.5-12.5) to 136 pM (118-147), n = 8 (median and interquartile range) and in duodenal ulcer patients, from 33 pM (21-52) to 213 pM (157-233), n = 7. The PP response to hypoglycemia was diminished by atropine in normal subjects (P < 0.005) and completely abolished by vagotomy in the duodenal ulcer patients. (b) Electrical stimulation, 8 Hz, of the vagal nerves in anesthetized pigs induced an increase in portal PP concentrations within 30 s from 32 pM (28-39) to 285 pM (248-294), n = 12. Minimal stimulatory frequency was 0.5 Hz and maximal stimulatory frequency 8-12 Hz. Atropine inhibited the PP response to electrical stimulation. Median inhibition with 0.5 mg of atropine/kg body wt was 74%, range 31-90%, n = 6. The response was eliminated by hexamethonium. Adrenergic alpha and beta blockade did not influence the release of PP in response to vagal stimulation. (c) Acetylcholine stimulated, in a dose-dependent manner, the secretion of PP from the isolated perfused porcine pancreas, half-maximal effective dose being 0.19 muM; maximal PP output in response to 5 min stimulation was 228 pmol, range 140-342 pmol, n = 5. Atropine completely abolished this response.The results of the present study together with the previously demonstrated poor PP response to food in vagotomized patients, indicate that vagal, cholinergic stimulation is a major regulator of PP secretion.
采用三种方法研究了传出性副交感神经刺激对胰多肽（PP）分泌的影响：（a）正常受试者和十二指肠溃疡患者在胰岛素诱导的低血糖状态下，血浆PP浓度均升高。正常受试者中，血浆PP浓度从11 pM（9.5 - 12.5）升高至136 pM（118 - 147），n = 8（中位数和四分位间距）；十二指肠溃疡患者中，血浆PP浓度从33 pM（21 - 52）升高至213 pM（157 - 233），n = 7。正常受试者中，阿托品可减弱低血糖引起的PP反应（P < 0.005）；十二指肠溃疡患者中，迷走神经切断术可完全消除该反应。（b）在麻醉猪中，以8 Hz频率电刺激迷走神经，可使门静脉PP浓度在30 s内从32 pM（28 - 39）升高至285 pM（248 - 294），n = 12。最小刺激频率为0.5 Hz，最大刺激频率为8 - 12 Hz。阿托品可抑制电刺激引起的PP反应。0.5 mg/kg体重的阿托品的中位抑制率为74%，范围为31% - 90%，n = 6。六甲铵可消除该反应。肾上腺素能α和β受体阻断剂不影响迷走神经刺激引起的PP释放。（c）乙酰胆碱以剂量依赖方式刺激离体灌注猪胰腺分泌PP，半数有效剂量为0.19 μM；5分钟刺激后的最大PP分泌量为228 pmol，范围为140 - 342 pmol，n = 5。阿托品可完全消除该反应。本研究结果以及先前证明的迷走神经切断术患者对食物的PP反应较差，表明迷走神经胆碱能刺激是PP分泌的主要调节因素。
