Snook J A, Lowes J R, Wu K C, Priddle J D, Jewell D P
Gastroenterology Department, Radcliffe Infirmary, Oxford.
Gut. 1991 Feb;32(2):163-6. doi: 10.1136/gut.32.2.163.
Sera and colonic tissue-bound immunoglobulin extracts from patients with ulcerative colitis and disease controls were examined immunohistochemically and by killer cell cytotoxicity assay for the presence of anticolonic epithelial autoantibodies. IgG yields in the tissue extracts from patients with colitis and control subjects were similar, and the extracts were uniformly autoantibody negative. Of 41 sera from patients with inflammatory bowel disease, 'classical' anticolon antibody was present in 41% and was commoner in patients with sclerosing cholangitis. Cytotoxic anticolon antibody was present in 20% overall and was strongly associated with disease activity; it did not correlate with the presence of 'classical' anticolon antibody. The heterogeneous and non-universal antiepithelial autoantibody response and the failure to detect tissue bound autoantibody in vivo argue against the hypothesis that humoral autoimmunity is of major importance in the pathogenesis of ulcerative colitis.
对溃疡性结肠炎患者和疾病对照者的血清及结肠组织结合免疫球蛋白提取物进行免疫组织化学检查，并通过杀伤细胞细胞毒性试验检测抗结肠上皮自身抗体的存在。结肠炎患者和对照受试者的组织提取物中的IgG产量相似，且提取物均为自身抗体阴性。在41份炎症性肠病患者的血清中，41%存在“经典”抗结肠抗体，在硬化性胆管炎患者中更为常见。细胞毒性抗结肠抗体总体上存在于20%的患者中，且与疾病活动密切相关；它与“经典”抗结肠抗体的存在无关。自身抗体反应的异质性和非普遍性，以及在体内未能检测到组织结合自身抗体，这与体液自身免疫在溃疡性结肠炎发病机制中起主要作用的假设相悖。
