Xu X, LoCicero J, Macri E, Loda M, Ellis F H
Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115, USA.
J Surg Res. 2000 Feb;88(2):120-4. doi: 10.1006/jsre.1999.5774.
The development of Barrett's esophagus (BE) and Barrett's associated adenocarcinoma (BAA) in the rat after experimental inducement of esophageal reflux of gastric, bile, and pancreatic juice has been reported by others. The purpose of this study was to determine whether similar results could be demonstrated in the mouse model.
One hundred eight Swiss-Webster mice were used in this study and were divided into three groups: Group I, 37 mice with esophagojejunostomy; Group II, 39 mice with esophagojejunostomy and the carcinogen N-methyl-N-benzylnitrosamine (MBN); and Group III, 32 mice with MBN alone. The animals were sacrificed after 19 weeks. Macroscopic and histopathologic examinations were performed.
One hundred mice survived and were available for pathologic study. Macroscopic evidence suggested esophagitis in 60.6% of mice in Group I, 62.8% of mice in Group II, and 9% of mice in Group III and suggested tumor in 3% of mice in Group I, 51.4% of mice in Group II, and 53.1% of mice in Group III. Histopathologic analysis disclosed BE in 42.4% of mice in Group I, 20% of mice in Group II, and 12.5% of mice in Group III. Cancer was present in 12.2% of mice in Group I, 54.3% of mice in Group II, and 46.9% of mice in Group III. Adenocarcinoma with or without squamous cell carcinoma was present in 6.1% of mice in Group I, 37.1% of mice in Group II, and 12.5% of mice in Group III.
Esophagojejunostomy plus MBN in the mouse results in BE, BAA, or both in 57.1% of animals, consistent with findings in the rat model after similar interventions.
其他人曾报道过在实验诱导大鼠出现胃、胆汁和胰液食管反流后，巴雷特食管（BE）和巴雷特相关腺癌（BAA）的发生情况。本研究的目的是确定在小鼠模型中是否能得到类似结果。
本研究使用了108只瑞士韦伯斯特小鼠，分为三组：第一组，37只进行食管空肠吻合术的小鼠；第二组，39只进行食管空肠吻合术并给予致癌物N-甲基-N-苄基亚硝胺（MBN）的小鼠；第三组，32只仅给予MBN的小鼠。19周后处死动物，进行大体和组织病理学检查。
100只小鼠存活并可用于病理研究。大体检查结果显示，第一组60.6%的小鼠、第二组62.8%的小鼠和第三组9%的小鼠有食管炎表现；第一组3%的小鼠、第二组51.4%的小鼠和第三组53.1%的小鼠有肿瘤表现。组织病理学分析发现，第一组42.4%的小鼠、第二组20%的小鼠和第三组12.5%的小鼠有BE。第一组12.2%的小鼠、第二组54.3%的小鼠和第三组46.9%的小鼠有癌症。第一组6.1%的小鼠、第二组37.1%的小鼠和第三组12.5%的小鼠有伴或不伴鳞状细胞癌的腺癌。
小鼠进行食管空肠吻合术加MBN处理后，57.1%的动物出现BE、BAA或两者皆有，这与类似干预后大鼠模型的结果一致。
