National Center for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, Italy.
Istituto Nazionale di Fisica Nucleare (INFN), Sezione di Roma1, Department of Physics, University La Sapienza, 00185 Rome, Italy.
Int J Mol Sci. 2022 Sep 5;23(17):10156. doi: 10.3390/ijms231710156.
This study investigated the peripheral blood lymphocytes (PBL) response to a dose of γ-rays in patients treated with radioiodine (I-131) for hyperthyroidism vs. healthy controls, to gain information about the individual lymphocytes' radio-sensitivity. Blood samples were taken from 18 patients and 10 healthy donors. Phosphorylated histone variant H2AX (γ-H2AX) and micronuclei (MN) induction were used to determine the change in PBL radio-sensitivity and the correlations between the two types of damage. The two assays showed large inter-individual variability in PBL background damage and in radio-sensitivity (patients vs. healthy donors). In particular, they showed an increased radio-sensitivity in 36% and 33% of patients, decrease in 36% and 44%, respectively. There was a scarce correlation between the two assays and no dependence on age or gender. A significant association was found between high radio-sensitivity conditions and induced hypothyroidism. PBL radio-sensitivity in the patient group was not significantly affected by treatment with I-131, whereas there were significant changes inter-individually. The association found between clinical response and PBL radio-sensitivity suggests that the latter could be used in view of the development of personalized treatments.
本研究调查了接受放射性碘（I-131）治疗甲状腺功能亢进症的患者与健康对照者外周血淋巴细胞（PBL）对剂量γ射线的反应，以了解个体淋巴细胞的放射敏感性。从 18 名患者和 10 名健康供体中采集血液样本。磷酸化组蛋白变体 H2AX（γ-H2AX）和微核（MN）诱导用于确定 PBL 放射敏感性的变化以及两种类型损伤之间的相关性。这两种检测方法在 PBL 背景损伤和放射敏感性方面均显示出个体间的巨大差异（患者与健康供体）。特别是，它们在 36%和 33%的患者中显示出增加的放射敏感性，而在 36%和 44%的患者中则降低了放射敏感性。两种检测方法之间相关性较差，与年龄或性别无关。高放射敏感性与诱导性甲状腺功能减退症之间存在显著相关性。患者组的 PBL 放射敏感性不受 I-131 治疗的显著影响，但个体间存在显著变化。在临床反应与 PBL 放射敏感性之间发现的相关性表明，鉴于个性化治疗的发展，可以使用后者。
