Furumitsu Y, Inaba M, Yukioka K, Yukioka M, Kumeda Y, Azuma Y, Ohta T, Ochi T, Nishizawa Y, Morii H
Yukioka Hospital, Second Department of Internal Medicine, Osaka City University Medical School, Japan.
J Rheumatol. 2000 Jan;27(1):64-70.
To elucidate the major source of pyridinium crosslinks in rheumatoid arthritis (RA).
Serum samples were collected from 75 patients with RA and 41 healthy controls, and synovial fluid (SF) samples were collected from 20 patients with RA and 13 with osteoarthritis (OA). Paired samples of serum and SF were collected at the same time from 26 patients with RA. Levels of pyridinium crosslinks were determined by a recently developed high sensitivity assay method using high pressure liquid chromatography.
The levels of serum pyridinoline (PYD) and serum deoxypyridinoline (DPD) were significantly higher in patients with RA than in healthy controls, and significantly correlated with laboratory variables indicating disease activity and severity. The levels of SF DPD, but not SF PYD, were significantly higher in patients with RA than in patients with OA. The levels of SF PYD and SF DPD both showed a significantly positive correlation with those of either SF interleukin 1beta or SF interleukin 6 in patients with RA. Finally, the levels of PYD, but not DPD, were higher in SF than in serum in all paired RA samples collected at the same time, with significant correlation between the members of each pair.
These observations suggest than an increase of PYD in RA serum may originate mostly from affected joints and that an increase of DPD in RA serum may be influenced more by systemic bone resorption.
阐明类风湿关节炎（RA）中吡啶交联物的主要来源。
收集75例RA患者和41例健康对照者的血清样本，以及20例RA患者和13例骨关节炎（OA）患者的滑液（SF）样本。同时从26例RA患者中收集配对的血清和SF样本。采用最近开发的高压液相色谱高灵敏度检测方法测定吡啶交联物水平。
RA患者血清吡啶啉（PYD）和血清脱氧吡啶啉（DPD）水平显著高于健康对照者，且与指示疾病活动度和严重程度的实验室指标显著相关。RA患者SF中DPD水平显著高于OA患者，而SF中PYD水平无显著差异。RA患者中，SF中PYD和SF中DPD水平均与SF白细胞介素1β或SF白细胞介素6水平呈显著正相关。最后，在同时收集的所有配对RA样本中，SF中PYD水平高于血清，而DPD水平则相反，且每对样本之间存在显著相关性。
这些观察结果表明，RA血清中PYD增加可能主要源于受累关节，而RA血清中DPD增加可能更多地受全身骨吸收影响。
