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多胺与解脂厌氧弧菌肽聚糖的共价连接。

Covalent linkage of polyamines to peptidoglycan in Anaerovibrio lipolytica.

作者信息

Hirao T, Sato M, Shirahata A, Kamio Y

机构信息

Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan.

出版信息

J Bacteriol. 2000 Feb;182(4):1154-7. doi: 10.1128/JB.182.4.1154-1157.2000.

Abstract

Spermidine and cadaverine were found to be constituents of the cell wall peptidoglycan of Anaerovibrio lipolytica, a strictly anaerobic bacterium. The peptidoglycan was degraded with the N-acetylmuramyl-L-alanine amidase and endopeptidase into two peptide fragments, peptide I and peptide II, at a molar ratio of 4:1. Peptides I and II were identified as L-alanine-D-glutamic acid(alphacadaverine)gammameso-diaminopimelic acid (DAP)-D-alanine and L-alanine-D-glutamic acid(alphaspermidine)gammameso-DAP-D-alanine, respectively. The N(1)-amino group of spermidine was linked to the alpha-carboxyl group of the D-glutamic acid residue of peptide II.

摘要

在严格厌氧细菌解脂厌氧弧菌(Anaerovibrio lipolytica)的细胞壁肽聚糖中发现了亚精胺和尸胺。肽聚糖被N - 乙酰胞壁酰 - L - 丙氨酸酰胺酶和内肽酶降解为两个肽片段,即肽I和肽II,摩尔比为4:1。肽I和肽II分别被鉴定为L - 丙氨酸 - D - 谷氨酸(α - 尸胺) - γ - 中 - 二氨基庚二酸(DAP) - D - 丙氨酸和L - 丙氨酸 - D - 谷氨酸(α - 亚精胺) - γ - 中 - DAP - D - 丙氨酸。亚精胺的N(1)-氨基与肽II的D - 谷氨酸残基的α - 羧基相连。

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