Papayannopoulou T H, Brice M, Stamatoyannopoulos G
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2033-7. doi: 10.1073/pnas.73.6.2033.
The regulation of fetal hemoglobin in adult erythroid cells was investigated with bone marrow cultures. Fetal hemoglobin (Hb F) was identified in individual erythroid colonies with fluorescent antibodies against Hb F and synthesis of gamma chains was determined with analyses of radioactive globins. The appearance of fetal hemoglobin in erythroid colonies was clonal. All the cells of the Hb F synthesizing colonies contained fetal hemoglobin. The frequency of erythroid colonies showing Hb F was higher than expected compared to the frequency of Hb F containing cells in the blood. Production of Hb F in culture, as shown by analysis of the radioactive globins, was 5 to 14 times higher than baseline Hb F synthesis. These results suggest that the ability for gamma chain synthesis in erythroid cells is determined at or above the level of the precursor cell from which the erythroid colonies, in vitro, derive (probably an erythropoietin responsive stem cell), and that stimulation of fetal hemoglobin synthesis in adult erythroid cells is possible.
利用骨髓培养研究了成人红系细胞中胎儿血红蛋白的调控。用抗胎儿血红蛋白的荧光抗体在单个红系集落中鉴定胎儿血红蛋白（Hb F），并通过分析放射性珠蛋白来测定γ链的合成。红系集落中胎儿血红蛋白的出现是克隆性的。合成Hb F的集落中的所有细胞都含有胎儿血红蛋白。与血液中含Hb F细胞的频率相比，显示Hb F的红系集落的频率高于预期。通过对放射性珠蛋白的分析表明，培养物中Hb F的产生比基线Hb F合成高5至14倍。这些结果表明，红系细胞中γ链合成的能力在体外红系集落所源自的前体细胞水平或更高水平上被确定（可能是对促红细胞生成素反应的干细胞），并且在成人红系细胞中刺激胎儿血红蛋白合成是可能的。
