组合方法在寻找类丝氨酸蛋白酶活性中的应用，重点关注酰基转移步骤。
Application of Combinatorial Procedures in the Search for Serine-Protease-Like Activity with Focus on the Acyl Transfer Step.
作者信息
Madder A, Farcy N, Pérez-Payán MN, Öhberg LM, Davis AP
机构信息
Laboratory for Organic Synthesis Department of Organic Chemistry, University of Gent Krijgslaan 281, 9000 Gent (Belgium).
出版信息
Angew Chem Int Ed Engl. 2000 Jan;39(1):145-148. doi: 10.1002/(sici)1521-3773(20000103)39:1<145::aid-anie145>3.0.co;2-j.
Recursive deconvolution of a 729-membered peptide library has identified three active sequences, in which both Ser and His are present in one of the two tripeptidic chains generated on a steroidal scaffold (see structural formula), for the cleavage of an activated p-nitrophenyl ester. This combinatorial approach aims at searching for serine-protease-like activity.
对一个由729个成员组成的肽库进行递归去卷积，已鉴定出三个活性序列，其中丝氨酸（Ser）和组氨酸（His）都存在于甾体支架上产生的两条三肽链之一中（见结构式），用于切割活化的对硝基苯酯。这种组合方法旨在寻找类丝氨酸蛋白酶活性。
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